Wolbachia and DNA Barcoding Insects: Patterns, Potential, and Problems

dc.contributor.authorSmith, M. A.
dc.contributor.authorBertrand, Claudia
dc.contributor.authorCrosby, Kate
dc.contributor.authorEveleigh, Eldon S.
dc.contributor.authorFernandez-Triana, Jose
dc.contributor.authorFisher, Brian L.
dc.contributor.authorGibbs, Jason
dc.contributor.authorHajibabaei, Mehrdad
dc.contributor.authorHallwachs, Winnie D.
dc.contributor.authorHind, Katharine
dc.contributor.authorHrcek, Jan
dc.contributor.authorHuang, Da-Wei
dc.contributor.authorJanda, Milan
dc.contributor.authorJanzen, Daniel H.
dc.contributor.authorLi, Yanwei
dc.contributor.authorMiller, Scott E.
dc.contributor.authorPacker, Lawrence
dc.contributor.authorQuicke, Donald L. J.
dc.contributor.authorRatnasingham, Sujeevan
dc.contributor.authorRodriguez, Josephine J.
dc.contributor.authorRougerie, Rodolphe
dc.contributor.authorShaw, Mark R.
dc.contributor.authorSheffield, Cory
dc.contributor.authorStahlhut, Julie K.
dc.contributor.authorSteinke, Dirk
dc.contributor.authorWhitfield, James B.
dc.contributor.authorWood, Monty
dc.contributor.authorZhou, Xin
dc.date.accessioned2013-04-01T16:44:24Z
dc.date.available2013-04-01T16:44:24Z
dc.date.issued2012
dc.description.abstractWolbachia is a genus of bacterial endosymbionts that impacts the breeding systems of their hosts. Wolbachia can confuse the patterns of mitochondrial variation, including DNA barcodes, because it influences the pathways through which mitochondria are inherited. We examined the extent to which these endosymbionts are detected in routine DNA barcoding, assessed their impact upon the insect sequence divergence and identification accuracy, and considered the variation present in Wolbachia COI. Using both standard PCR assays (Wolbachia surface coding protein – wsp), and bacterial COI fragments we found evidence of Wolbachia in insect total genomic extracts created for DNA barcoding library construction. When >2 million insect COI trace files were examined on the Barcode of Life Datasystem (BOLD) Wolbachia COI was present in 0.16% of the cases. It is possible to generate Wolbachia COI using standard insect primers; however, that amplicon was never confused with the COI of the host. Wolbachia alleles recovered were predominantly Supergroup A and were broadly distributed geographically and phylogenetically. We conclude that the presence of the Wolbachia DNA in total genomic extracts made from insects is unlikely to compromise the accuracy of the DNA barcode library; in fact, the ability to query this DNA library (the database and the extracts) for endosymbionts is one of the ancillary benefits of such a large scale endeavor – for which we provide several examples. It is our conclusion that regular assays for Wolbachia presence and type can, and should, be adopted by large scale insect barcoding initiatives. While COI is one of the five multi-locus sequence typing (MLST) genes used for categorizing Wolbachia, there is limited overlap with the eukaryotic DNA barcode region.
dc.format.extente36514
dc.identifier1932-6203
dc.identifier.citationSmith, M. A., Bertrand, Claudia, Crosby, Kate, Eveleigh, Eldon S., Fernandez-Triana, Jose, Fisher, Brian L., Gibbs, Jason, Hajibabaei, Mehrdad, Hallwachs, Winnie D., Hind, Katharine, Hrcek, Jan, Huang, Da-Wei, Janda, Milan, Janzen, Daniel H., Li, Yanwei, Miller, Scott E., Packer, Lawrence, Quicke, Donald L. J., Ratnasingham, Sujeevan, Rodriguez, Josephine J., Rougerie, Rodolphe, Shaw, Mark R., Sheffield, Cory, Stahlhut, Julie K., Steinke, Dirk et al. 2012. "<a href="https://repository.si.edu/handle/10088/20428">Wolbachia and DNA Barcoding Insects: Patterns, Potential, and Problems</a>." <em>PloS ONE</em>, 7, (5) e36514. <a href="https://doi.org/10.1371/journal.pone.0036514">https://doi.org/10.1371/journal.pone.0036514</a>.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10088/20428
dc.publisherPublic Library of Science
dc.relation.ispartofPloS ONE 7 (5)
dc.titleWolbachia and DNA Barcoding Insects: Patterns, Potential, and Problems
dc.typearticle
sro.description.unitNMNH
sro.description.unitNH-Entomology
sro.identifier.doi10.1371/journal.pone.0036514
sro.identifier.itemID114578
sro.identifier.refworksID83850
sro.identifier.urlhttps://repository.si.edu/handle/10088/20428
sro.publicationPlaceSan Francisco

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