Isolation and characterization of microsatellite loci in the common milkweed, Asclepias syriaca (Apocynaceae)

Abstract

Premise of the study: Microsatellite primers were developed for the common milkweed, Asclepias syriaca L., to assist in genet identification and the analysis of spatial genetic structure. * Methods and Results: Using an enrichment cloning protocol, eight microsatellite loci were isolated and characterized in a Michigan population of A. syriaca. The primers amplified di- and trinucleotide repeats with 4-13 alleles per locus. * Conclusions: The primers will be useful for studies of clonality and gene flow in natural populations.