Unexpected molecular and morphological diversity of hemichordate larvae from the Neotropics

dc.contributor.authorCollin, Rachel
dc.contributor.authorVenera‐Pontón, Dagoberto E.
dc.contributor.authorDriskell, Amy C.
dc.contributor.authorMacdonald, Kenneth S.
dc.contributor.authorBoyle, Michael J.
dc.date.accessioned2019-12-12T03:01:13Z
dc.date.available2019-12-12T03:01:13Z
dc.date.issued2019
dc.description.abstractThe diversity of tropical marine invertebrates is poorly documented, especially those groups for which collecting adults is difficult. We collected the planktonic tornaria larvae of hemichordates (acorn worms) to assess their hidden diversity in the Neotropics. Larvae were retrieved in plankton tows from waters of the Pacific and Caribbean coasts of Panama, followed by DNA barcoding of mitochondrial cy- tochrome c oxidase subunit I (COI) and 16S ribosomal DNA to estimate their diversity in the region. With moderate sampling efforts, we discovered six operational taxo- nomic units (OTUs) in the Bay of Panama on the Pacific coast, in contrast to the single species previously recorded for the entire Tropical Eastern Pacific. We found eight OTUs in Bocas del Toro province on the Caribbean coast, compared to seven spe- cies documented from adults in the entire Caribbean. All OTUs differed from each other and from named acorn worm sequences in GenBank by >10% pairwise distance in COI and >2% in 16S. Two of our OTUs matched 16S hemichordate sequences in GenBank: one was an unidentified or unnamed Balanoglossus from the Caribbean of Panama, and the other was an unidentified ptychoderid larva from the Bahamas. The species accumulation curves suggest that nearly all the species have been collected and only one more species might still remain undetected in the Pacific. In contrast, the Caribbean species accumulation curve suggests that further sampling could yield more than 10 additional OTUs. Tornaria from the 14 OTUs exhibited typical planktotrophic morphologies, and, in some cases, may be distinguished by differences in pigmentation and by the number of telotrochal ciliary bands, but in general, few diagnostic differences were detected.
dc.format.extentArticle e12273
dc.identifier1077-8306
dc.identifier.citationCollin, Rachel, Venera‐Pontón, Dagoberto E., Driskell, Amy C., Macdonald, Kenneth S., and Boyle, Michael J. 2019. "<a href="https://repository.si.edu/handle/100889/99848">Unexpected molecular and morphological diversity of hemichordate larvae from the Neotropics</a>." <em>Invertebrate Biology</em>, 138, (4) Article e12273. <a href="https://doi.org/10.1111/ivb.12273">https://doi.org/10.1111/ivb.12273</a>.
dc.identifier.issn1077-8306
dc.identifier.urihttps://hdl.handle.net/100889/99848
dc.publisherWiley
dc.relation.ispartofInvertebrate Biology 138 (4)
dc.titleUnexpected molecular and morphological diversity of hemichordate larvae from the Neotropics
dc.typearticle
sro.description.unitNMNH
sro.description.unitSTRI
sro.description.unitNH-SMS
sro.description.unitNH-Other
sro.identifier.doi10.1111/ivb.12273
sro.identifier.itemID153310
sro.identifier.refworksID37175
sro.identifier.urlhttps://repository.si.edu/handle/100889/99848
sro.publicationPlaceHoboken, New Jersey

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Collin_Rachel-20191104-Collin_et_al_Hemichordate_Revised_Sept21.pdf
Size:
385.56 KB
Format:
Adobe Portable Document Format
Description: