Methodological considerations for monitoring soil/litter arthropods in tropical rainforests using DNA metabarcoding, with a special emphasis on ants, springtails and termites
dc.contributor.author | Basset, Yves | |
dc.contributor.author | Donoso, David A. | |
dc.contributor.author | Hajibabaei, Mehrdad | |
dc.contributor.author | Wright, Michael T. G. | |
dc.contributor.author | Perez, Kate H. J. | |
dc.contributor.author | Lamarre, Greg P. A. | |
dc.contributor.author | De León, Luis F. | |
dc.contributor.author | Palacios-Vargas, Jos | |
dc.contributor.author | Castaño-Meneses, Gabriela | |
dc.contributor.author | Rivera, Marleny | |
dc.contributor.author | Perez, Filonila | |
dc.contributor.author | Bobadilla, Ricardo | |
dc.contributor.author | Lopez, Yacksecari | |
dc.contributor.author | Ramirez, José Alejandro | |
dc.contributor.author | Barrios, Héctor | |
dc.date.accessioned | 2021-12-07T02:32:24Z | |
dc.date.available | 2021-12-07T02:32:24Z | |
dc.date.issued | 2020 | |
dc.description.abstract | Robust data to refute or support claims of global insect decline are currently lacking, particularly for the soil fauna in the tropics. DNA metabarcoding represents a powerful approach for rigorous spatial and temporal monitoring of the taxonomically challenging soil fauna. Here, we provide a detailed field protocol, which was successfully applied in Barro Colorado Island (BCI) in Panama, to collect soil samples and arthropods in a tropical rainforest, to be later processed with metabarcoding. We also estimate the proportion of soil/litter ant, springtail and termite species from the local fauna that can be detected by metabarcoding samples obtained either from Berlese-Tullgren (soil samples), Malaise or light traps. Each collecting method detected a rather distinct fauna. Soil and Malaise trap samples detected 213 species (73%) of all target species. Malaise trap samples detected many ant species, whereas soil samples were more efficient at detecting springtail and termite species. With respect to long-term monitoring of soil-dwelling and common species (more amenable to statistical trends), the best combination of two methods were soil and light trap samples, detecting 94% of the total of common species. A protocol including 100 soil, 40 Malaise and 80 light trap samples annually processed by metabarcoding would allow the long-term monitoring of at least 11%, 18% and 16% of species of soil/litter ants, springtails and termites, respectively, present on BCI, and a high proportion of the total abundance (up to 80% of all individuals) represented by these taxa. | |
dc.identifier | 2534-9708 | |
dc.identifier.citation | Basset, Yves, Donoso, David A., Hajibabaei, Mehrdad, Wright, Michael T. G., Perez, Kate H. J., Lamarre, Greg P. A., De León, Luis F., Palacios-Vargas, Jos, Castaño-Meneses, Gabriela, Rivera, Marleny, Perez, Filonila, Bobadilla, Ricardo, Lopez, Yacksecari, Ramirez, José Alejandro, and Barrios, Héctor. 2020. "<a href="https://repository.si.edu/handle/10088/111912">Methodological considerations for monitoring soil/litter arthropods in tropical rainforests using DNA metabarcoding, with a special emphasis on ants, springtails and termites</a>." <em>Metabarcoding and Metagenomics</em>, 4. <a href="https://doi.org/10.3897/mbmg.4.58572">https://doi.org/10.3897/mbmg.4.58572</a>. | |
dc.identifier.issn | 2534-9708 | |
dc.identifier.uri | https://hdl.handle.net/10088/111912 | |
dc.relation.ispartof | Metabarcoding and Metagenomics 4 | |
dc.title | Methodological considerations for monitoring soil/litter arthropods in tropical rainforests using DNA metabarcoding, with a special emphasis on ants, springtails and termites | |
dc.type | article | |
sro.description.unit | STRI | |
sro.identifier.doi | 10.3897/mbmg.4.58572 | |
sro.identifier.itemID | 161199 | |
sro.identifier.refworksID | 25949 | |
sro.identifier.url | https://repository.si.edu/handle/10088/111912 |
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