How I wonder what you are: Can DNA barcoding identify the larval asteroids of Panama?

dc.contributor.authorCollin, Rachel
dc.contributor.authorVenera-Ponton, Dagoberto E.
dc.contributor.authorDriskell, Amy C.
dc.contributor.authorMacdonald, Kenneth S.
dc.contributor.authorBoyle, Michael J.
dc.date.accessioned2021-01-14T19:13:27Z
dc.date.available2021-01-14T19:13:27Z
dc.date.issued2020
dc.description.abstractAs part of a project to document the diversity of larval invertebrates on both coasts of Panama, we collected and photographed 141 larval asteroids and sequenced fragments of their mitochondrial cytochrome c oxidase subunit I (COI) and 16S ribosomal DNA. We uncovered 10 Caribbean operational taxonomic units (OTUs) and five Pacific OTUs. We could identify six of the 15 OTUs based on >99% similarity with reference sequences in GenBank: The Pacific species Astropecten verrilli and Pentaceraster cumingi and the Caribbean species Astropecten marginatus, Astropecten antillensis, Oreaster reticulatus, and Mithrodia clavigera. Two other OTUs were placed in BINs in the Barcode of Life Database (BOLD) with unpublished sequences that were identified as Pharia pyramidata from the Pacific and Valvaster striatus, now known from the Caribbean as well as the Indo-West Pacific. The remaining seven species appear likely to belong to Luidia, as 16S sequences from each have 87%-95% identity with various species of Luidia, and the sequences nest among species of Luidia in neighbor-joining trees. The low diversity of asteroid larvae reflects similar to 10% of the diversity of adult sea stars reported from Panama, and highlights the need for broader collection efforts and improved coverage of DNA barcode reference sequences for Luidia and other soft-bottom species.
dc.format.extente12303–e12303
dc.identifier1077-8306
dc.identifier.citationCollin, Rachel, Venera-Ponton, Dagoberto E., Driskell, Amy C., Macdonald, Kenneth S., and Boyle, Michael J. 2020. "<a href="https://repository.si.edu/handle/10088/108851">How I wonder what you are: Can DNA barcoding identify the larval asteroids of Panama?</a>" <em>Invertebrate Biology</em>, e12303–e12303. <a href="https://doi.org/10.1111/ivb.12303">https://doi.org/10.1111/ivb.12303</a>.
dc.identifier.issn1077-8306
dc.identifier.urihttps://hdl.handle.net/10088/108851
dc.publisherWiley
dc.relation.ispartofInvertebrate Biology
dc.titleHow I wonder what you are: Can DNA barcoding identify the larval asteroids of Panama?
dc.typearticle
sro.description.unitNMNH
sro.description.unitNH-Other
sro.description.unitSTRI
sro.description.unitNH-SMS
sro.identifier.doi10.1111/ivb.12303
sro.identifier.itemID157932
sro.identifier.refworksID37172
sro.identifier.urlhttps://repository.si.edu/handle/10088/108851
sro.publicationPlaceHoboken, New Jersey

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