Effect of donor age on success of spermatogenesis in feline testis xenografts

dc.contributor.authorKim, Y.
dc.contributor.authorSelvaraj, V.
dc.contributor.authorPukazhenthi, Budhan S.
dc.contributor.authorTravis, A. J.
dc.date.accessioned2009-08-17T19:35:23Z
dc.date.available2009-08-17T19:35:23Z
dc.date.issued2007
dc.description.abstractEctopic xenografting of 'donor' feline testicular tissue into a 'recipient' immunodeficient mouse is a promising tool to preserve the male genome from genetically valuable felids. To define parameters under which the technique can succeed, we compared the effect of donor age on xenograft spermatogenesis among four age groups of domestic cats (Felis catus; age range 8 weeks to 15 months). In all cases, fresh tissue was grafted into castrated mice and collected 10, 30 and 50 weeks later. The percentage of xenografts recovered decreased as donor age increased. Mature testicular spermatozoa were observed in xenografts from the 8 and 9-16 week age groups; only a single 7-month-old donor produced elongating spermatids and xenografts from donors >/= 8 months of age degenerated. Seminal vesicle weight, an indicator of bioactive testosterone, was not significantly different between donors aged 8 weeks to 7 months and controls, suggesting that xenograft Leydig cells were ultimately functional even in the 5-7 month age group. Regardless of donor age, production of mature spermatozoa from xenografts was markedly delayed compared with controls. Comparison of xenografts that produced sperm with normal controls revealed a decrease in tubule cross-sections having post-meiotic germ cells. Together, these results indicate that the maximum practical donor age was just before the onset of puberty and that even successful xenografts had abnormalities in spermatogenesis.
dc.format.extent989296 bytes
dc.format.extent869–876
dc.format.mimetypeapplication/pdf
dc.identifier1031-3613
dc.identifier.citationKim, Y., Selvaraj, V., Pukazhenthi, Budhan S., and Travis, A. J. 2007. "<a href="https://repository.si.edu/handle/10088/7973">Effect of donor age on success of spermatogenesis in feline testis xenografts</a>." <em>Reproduction, Fertility, and Development</em>, 19, (7) 869–876.
dc.identifier.issn1031-3613
dc.identifier.urihttp://hdl.handle.net/10088/7973
dc.language.isoen_US
dc.relation.ispartofReproduction, Fertility, and Development 19 (7)
dc.titleEffect of donor age on success of spermatogenesis in feline testis xenografts
dc.typearticle
sro.description.unitNZP
sro.identifier.itemID79777
sro.identifier.refworksID48211
sro.identifier.urlhttps://repository.si.edu/handle/10088/7973
sro.publicationPlaceAustralia

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
1062C49D-42EE-4692-BF93-4FFC4274EC8F.pdf
Size:
966.11 KB
Format:
Adobe Portable Document Format