DNA Barcoding of <I>Rhodiola</I> (Crassulaceae): a Case Study on a Group of Recently Diversified Medicinal Plants from the Qinghai-Tibetan Plateau

dc.contributor.authorZhang, Jian-Qiang
dc.contributor.authorMeng, Shi-Yong
dc.contributor.authorWen, Jun
dc.contributor.authorRao, Guang-Yuan
dc.date.accessioned2015-04-20T15:15:19Z
dc.date.available2015-04-20T15:15:19Z
dc.date.issued2015
dc.description.abstractDNA barcoding, the identification of species using one or a few short standardized DNA sequences, is an important complement to traditional taxonomy. However, there are particular challenges for barcoding plants, especially for species with complex evolutionary histories. We herein evaluated the utility of five candidate sequences - rbcL, matK, trnH-psbA, trnL-F and the internal transcribed spacer (ITS) - for barcoding Rhodiola species, a group of high-altitude plants frequently used as adaptogens, hemostatics and tonics in traditional Tibetan medicine. Rhodiola was suggested to have diversified rapidly recently. The genus is thus a good model for testing DNA barcoding strategies for recently diversified medicinal plants. This study analyzed 189 accessions, representing 47 of the 55 recognized Rhodiola species in the Flora of China treatment. Based on intraspecific and interspecific divergence and degree of monophyly statistics, ITS was the best single-locus barcode, resolving 66% of the Rhodiola species. The core combination rbcL+matK resolved only 40.4% of them. Unsurprisingly, the combined use of all five loci provided the highest discrimination power, resolving 80.9% of the species. However, this is weaker than the discrimination power generally reported in barcoding studies of other plant taxa. The observed complications may be due to the recent diversification, incomplete lineage sorting and reticulate evolution of the genus. These processes are common features of numerous plant groups in the high-altitude regions of the Qinghai-Tibetan Plateau.
dc.format.extent1–15
dc.identifier1932-6203
dc.identifier.citationZhang, Jian-Qiang, Meng, Shi-Yong, Wen, Jun, and Rao, Guang-Yuan. 2015. "<a href="http://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0119921&representation=PDF">DNA Barcoding of <I>Rhodiola</I> (Crassulaceae): a Case Study on a Group of Recently Diversified Medicinal Plants from the Qinghai-Tibetan Plateau</a>." <em>PloS One</em>, 10, (3) 1–15. <a href="https://doi.org/10.1371/journal.pone.0119921">https://doi.org/10.1371/journal.pone.0119921</a>.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10088/25186
dc.identifier.urihttp://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0119921&representation=PDF
dc.publisherPublic Library of Science
dc.relation.ispartofPloS One 10 (3)
dc.titleDNA Barcoding of <I>Rhodiola</I> (Crassulaceae): a Case Study on a Group of Recently Diversified Medicinal Plants from the Qinghai-Tibetan Plateau
dc.typearticle
sro.description.unitNH-Botany
sro.description.unitNMNH
sro.identifier.doi10.1371/journal.pone.0119921
sro.identifier.itemID135534
sro.identifier.refworksID100877
sro.identifier.urlhttp://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0119921&representation=PDF
sro.publicationPlaceSan Francisco

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