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Cilostamide and forskolin maintain gap junction function of incubated dog follicles

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dc.contributor.author Thongkittidilok, Chommanart en
dc.contributor.author Doriguzzi, Nicole en
dc.contributor.author Nagashima, Jennifer en
dc.contributor.author Brown, Megan en
dc.contributor.author Chansaenroj, Ajjima en
dc.contributor.author Songsasen, Nucharin en
dc.date.accessioned 2019-10-23T02:01:36Z
dc.date.available 2019-10-23T02:01:36Z
dc.date.issued 2019
dc.identifier.citation Thongkittidilok, Chommanart, Doriguzzi, Nicole, Nagashima, Jennifer, Brown, Megan, Chansaenroj, Ajjima, and Songsasen, Nucharin. 2019. "<a href="http://www.ncbi.nlm.nih.gov/pubmed/31629307">Cilostamide and forskolin maintain gap junction function of incubated dog follicles</a>." <em>Theriogenology</em>. 142:222&ndash;228. <a href="https://doi.org/10.1016/j.theriogenology.2019.09.034">https://doi.org/10.1016/j.theriogenology.2019.09.034</a> en
dc.identifier.issn 1879-3231
dc.identifier.uri https://hdl.handle.net/10088/99183
dc.description.abstract Disruption of the communication between the oocyte and granulosa cells is one of the major causes of poor development of in vitro grown ovarian follicles and oocytes. The present study investigated the effect of two cAMP modulators, cilostamide and forskolin, on in vitro growth of isolated dog secondary follicles and enclosed oocytes, communication between the gamete and surrounding granulosa cells, expression of GJA1 and GDF9, as well as cAMP level. Secondary follicles were incubated with cilostamide or forskolin alone or a combination of 20 µM cilostamide +1 µM forskolin, and the diameter of the incubated follicles and enclosed oocytes assessed every 72 h. Gap junction activity, GJA1 and GDF9 expression and cAMP level were assessed on Days 6 and 12 and transzonal projection (TZP) density was evaluated on Day 12. Neither cilostamide nor forskolin alone enhanced in vitro growth of dog follicles and the enclosed oocytes (P?&gt;?0.05). However, these two cAMP modulators dose dependently sustained gap junction activity and stimulated cAMP production compared with the non-supplemented control. Cilostamide at the high dosage (20?µM) also upregulated GJA1 expression. The combination of cilostamide and forskolin supported oocyte growth during the first 9 days and upregulated GJA1 and GDF9 expression at Day 12 of in vitro culture. This combination treatment also sustained gap junction activity, cAMP production, and increased TZP function (calcein intensity: TZP density ratio). The findings indicated that a combination of cilostamide and forskolin supported growth and survival of oocytes enclosed within cultured follicles by sustaining cAMP production and gap junction activity. en
dc.relation.ispartof Theriogenology en
dc.title Cilostamide and forskolin maintain gap junction function of incubated dog follicles en
dc.type Journal Article en
dc.identifier.srbnumber 152766
dc.identifier.doi 10.1016/j.theriogenology.2019.09.034
rft.jtitle Theriogenology
rft.volume 142
rft.spage 222
rft.epage 228
dc.description.SIUnit NZP en
dc.description.SIUnit Peer-reviewed en
dc.citation.spage 222
dc.citation.epage 228
dc.relation.url http://www.ncbi.nlm.nih.gov/pubmed/31629307


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