Use and advantages of ethanol solution of alizarin red S dye for staining bone in fishes

Springer, Victor G.; Johnson, G. David

dc.contributor.author	Springer, Victor G.	en
dc.contributor.author	Johnson, G. David	en
dc.date.accessioned	2010-06-29T17:37:50Z
dc.date.available	2010-06-29T17:37:50Z
dc.date.issued	2000
dc.identifier.citation	Springer, Victor G. and Johnson, G. David. 2000. "<a href="https://repository.si.edu/handle/10088/9783">Use and advantages of ethanol solution of alizarin red S dye for staining bone in fishes</a>." <em>Copeia</em>. 300–301.	en
dc.identifier.issn	0045-8511
dc.identifier.uri	http://hdl.handle.net/10088/9783
dc.description.abstract	A solution of 75% ethyl alcohol (ETOH) and alizarin red S can be substituted for KOH-alizarin red S to stain bone. ETOH-alizarin, unlike KOH-alizarin, does not damage muscle or cartilage. ETOH-alizarin and acidified alcian blue (a cartilage stain) can be used in any order and as often as required to stain bone and cartilage in a single preparation that has not been exposed to KOH-alizarin or trypsin, remembering that the acidic effects of the alcian blue solution should be neutralized to prevent decalcification of weakly ossified elements.	en
dc.relation.ispartof	Copeia	en
dc.title	Use and advantages of ethanol solution of alizarin red S dye for staining bone in fishes	en
dc.type	Journal Article	en
dc.identifier.srbnumber	74609
rft.jtitle	Copeia
rft.issue	1
rft.spage	300
rft.epage	301
dc.description.SIUnit	NMNH	en
dc.description.SIUnit	NH-Vertebrate Zoology	en
dc.citation.spage	300
dc.citation.epage	301