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Use and advantages of ethanol solution of alizarin red S dye for staining bone in fishes

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dc.contributor.author Springer, Victor G. en
dc.contributor.author Johnson, G. David en
dc.date.accessioned 2010-06-29T17:37:50Z
dc.date.available 2010-06-29T17:37:50Z
dc.date.issued 2000
dc.identifier.citation Springer, Victor G. and Johnson, G. David. 2000. "<a href="https://repository.si.edu/handle/10088/9783">Use and advantages of ethanol solution of alizarin red S dye for staining bone in fishes</a>." <em>Copeia</em>. 300&ndash;301. en
dc.identifier.issn 0045-8511
dc.identifier.uri http://hdl.handle.net/10088/9783
dc.description.abstract A solution of 75% ethyl alcohol (ETOH) and alizarin red S can be substituted for KOH-alizarin red S to stain bone. ETOH-alizarin, unlike KOH-alizarin, does not damage muscle or cartilage. ETOH-alizarin and acidified alcian blue (a cartilage stain) can be used in any order and as often as required to stain bone and cartilage in a single preparation that has not been exposed to KOH-alizarin or trypsin, remembering that the acidic effects of the alcian blue solution should be neutralized to prevent decalcification of weakly ossified elements. en
dc.relation.ispartof Copeia en
dc.title Use and advantages of ethanol solution of alizarin red S dye for staining bone in fishes en
dc.type Journal Article en
dc.identifier.srbnumber 74609
rft.jtitle Copeia
rft.issue 1
rft.spage 300
rft.epage 301
dc.description.SIUnit NMNH en
dc.description.SIUnit NH-Vertebrate Zoology en
dc.citation.spage 300
dc.citation.epage 301


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