Use and advantages of ethanol solution of alizarin red S dye for staining bone in fishes

Springer, Victor G.; Johnson, G. David

dc.contributor.author	Springer, Victor G.
dc.contributor.author	Johnson, G. David
dc.date.accessioned	2010-06-29T17:37:50Z
dc.date.available	2010-06-29T17:37:50Z
dc.date.issued	2000
dc.identifier	0045-8511
dc.identifier.citation	Springer, Victor G. and Johnson, G. David. 2000. "<a href="https://repository.si.edu/handle/10088/9783">Use and advantages of ethanol solution of alizarin red S dye for staining bone in fishes</a>." <em>Copeia</em>, (1) 300–301.
dc.identifier.issn	0045-8511
dc.identifier.uri	http://hdl.handle.net/10088/9783
dc.description.abstract	A solution of 75% ethyl alcohol (ETOH) and alizarin red S can be substituted for KOH-alizarin red S to stain bone. ETOH-alizarin, unlike KOH-alizarin, does not damage muscle or cartilage. ETOH-alizarin and acidified alcian blue (a cartilage stain) can be used in any order and as often as required to stain bone and cartilage in a single preparation that has not been exposed to KOH-alizarin or trypsin, remembering that the acidic effects of the alcian blue solution should be neutralized to prevent decalcification of weakly ossified elements.
dc.format.extent	300–301
dc.publisher	AMER SOC ICHTHYOLOGISTS HERPETOLOGISTS
dc.relation.ispartof	Copeia (1)
dc.title	Use and advantages of ethanol solution of alizarin red S dye for staining bone in fishes
dc.type	article
sro.identifier.refworksID	85653
sro.identifier.itemID	74609
sro.description.unit	NMNH
sro.description.unit	NH-Vertebrate Zoology
sro.identifier.url	https://repository.si.edu/handle/10088/9783
sro.publicationPlace	CHARLESTON; UNIV CHARLESTON, GRICE MARINE LABORATORY, 205 FORT JOHNSON RD, CHARLESTON, SC 29412 USA