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Assessing Metagenomic Signals Recovered from Lyuba, a 42,000-Year-Old Permafrost-Preserved Woolly Mammoth Calf

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dc.contributor.author Ferrari, Giada
dc.contributor.author Lischer, Heidi E. L.
dc.contributor.author Neukamm, Judith
dc.contributor.author Rayo, Enrique
dc.contributor.author Borel, Nicole
dc.contributor.author Pospischil, Andreas
dc.contributor.author Rühli, Frank
dc.contributor.author Bouwman, Abigail S.
dc.contributor.author Campana, Michael G.
dc.date.accessioned 2018-09-07T13:43:53Z
dc.date.available 2018-09-07T13:43:53Z
dc.date.issued 2018
dc.identifier 2073-4425
dc.identifier.citation Ferrari, Giada, Lischer, Heidi E. L., Neukamm, Judith, Rayo, Enrique, Borel, Nicole, Pospischil, Andreas, Rühli, Frank, Bouwman, Abigail S., and Campana, Michael G. 2018. "<a href="https://repository.si.edu/handle/10088/94161">Assessing Metagenomic Signals Recovered from Lyuba, a 42,000-Year-Old Permafrost-Preserved Woolly Mammoth Calf</a>." <em>Genes</em>, 9, (9). <a href="https://doi.org/10.3390/genes9090436">https://doi.org/10.3390/genes9090436</a>.
dc.identifier.issn 2073-4425
dc.identifier.uri http://www.mdpi.com/2073-4425/9/9/436
dc.identifier.uri https://hdl.handle.net/10088/94161
dc.description.abstract The reconstruction of ancient metagenomes from archaeological material, and their implication in human health and evolution, is one of the most recent advances in paleomicrobiological studies. However, as for all ancient DNA (aDNA) studies, environmental and laboratory contamination need to be specifically addressed. Here we attempted to reconstruct the tissue-specific metagenomes of a 42,000-year-old, permafrost-preserved woolly mammoth calf through shotgun high-throughput sequencing. We analyzed the taxonomic composition of all tissue samples together with environmental and non-template experimental controls and compared them to metagenomes obtained from permafrost and elephant fecal samples. Preliminary results suggested the presence of tissue-specific metagenomic signals. We identified bacterial species that were present in only one experimental sample, absent from controls, and consistent with the nature of the samples. However, we failed to further authenticate any of these signals and conclude that, even when experimental samples are distinct from environmental and laboratory controls, this does not necessarily indicate endogenous presence of ancient host-associated microbiomic signals.
dc.publisher Molecular Diversity Preservation International (MDPI)
dc.relation.ispartof Genes 9 (9)
dc.title Assessing Metagenomic Signals Recovered from Lyuba, a 42,000-Year-Old Permafrost-Preserved Woolly Mammoth Calf
dc.type article
sro.identifier.refworksID 12970
sro.identifier.itemID 148440
sro.description.unit NZP
sro.identifier.doi 10.3390/genes9090436
sro.identifier.url https://repository.si.edu/handle/10088/94161
sro.publicationPlace Basel, Switzerland


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