dc.contributor.author |
Sedio, Brian E. |
en |
dc.contributor.author |
Cristopher, Boya P., |
en |
dc.contributor.author |
Rojas Echeverri, Juan Camilo |
en |
dc.date.accessioned |
2018-06-02T09:01:24Z |
|
dc.date.available |
2018-06-02T09:01:24Z |
|
dc.date.issued |
2018 |
|
dc.identifier.citation |
Sedio, Brian E., Cristopher, Boya P.,, and Rojas Echeverri, Juan Camilo. 2018. "A protocol for high-throughput, untargeted forest community metabolomics using mass spectrometry molecular networks." <em>Applications in Plant Sciences</em>. 6 (3):<a href="https://doi.org/10.1002/aps3.1033">https://doi.org/10.1002/aps3.1033</a> |
en |
dc.identifier.issn |
2168-0450 |
|
dc.identifier.uri |
https://hdl.handle.net/10088/35677 |
|
dc.description.abstract |
Premise of the Study: We describe a field collection, sample processing, and ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) instrumental and bioinformatics method developed for untargeted metabolomics of plant tissue and suitable for molecular networking applications. Methods and Results: A total of 613 leaf samples from 204 tree species was collected in the field and analyzed using UHPLC-MS/MS. Matching of molecular fragmentation spectra generated over 125,000 consensus spectra representing unique molecular structures, 26,410 of which were linked to at least one structurally similar compound. Conclusions: Our workflow is able to generate molecular networks of hundreds of thousands of compounds representing broad classes of plant secondary chemistry and a wide range of molecular masses, from 100 to 2500 daltons, making possible large-scale comparative metabolomics, as well as studies of chemical community ecology and macroevolution in plants. |
en |
dc.relation.ispartof |
Applications in Plant Sciences |
en |
dc.title |
A protocol for high-throughput, untargeted forest community metabolomics using mass spectrometry molecular networks |
en |
dc.type |
Journal Article |
en |
dc.identifier.srbnumber |
146535 |
|
dc.identifier.doi |
10.1002/aps3.1033 |
|
rft.jtitle |
Applications in Plant Sciences |
|
rft.volume |
6 |
|
rft.issue |
3 |
|
dc.description.SIUnit |
STRI |
en |
dc.description.SIUnit |
Peer-reviewed |
en |