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Cryopreservation of Fish Spermatogonial Cells: The Future of Natural History Collections

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dc.contributor.author Hagedorn, Mary M. en
dc.contributor.author Daly, Jonathan P. en
dc.contributor.author Carter, Virginia L. en
dc.contributor.author Cole, Kathleen S. en
dc.contributor.author Jaafar, Zeehan en
dc.contributor.author Lager, Claire V. A. en
dc.contributor.author Parenti, Lynne R. en
dc.date.accessioned 2018-05-04T09:01:10Z
dc.date.available 2018-05-04T09:01:10Z
dc.date.issued 2018
dc.identifier.citation Hagedorn, Mary M., Daly, Jonathan P., Carter, Virginia L., Cole, Kathleen S., Jaafar, Zeehan, Lager, Claire V. A., and Parenti, Lynne R. 2018. "<a href="https://repository.si.edu/handle/10088/35556">Cryopreservation of Fish Spermatogonial Cells: The Future of Natural History Collections</a>." <em>Scientific Reports</em>. 8:6149&ndash;6149. <a href="https://doi.org/10.1038/s41598-018-24269-3">https://doi.org/10.1038/s41598-018-24269-3</a> en
dc.identifier.issn 2045-2322
dc.identifier.uri https://hdl.handle.net/10088/35556
dc.description.abstract As global biodiversity declines, the value of biological collections increases. Cryopreserved diploid spermatogonial cells meet two goals: to yield high-quality molecular sequence data; and to regenerate new individuals, hence potentially countering species extinction. Cryopreserved spermatogonial cells that allow for such mitigative measures are not currently in natural history museum collections because there are no standard protocols to collect them. Vertebrate specimens, especially fishes, are traditionally formalin-fixed and alcohol-preserved which makes them ideal for morphological studies and as museum vouchers, but inadequate for molecular sequence data. Molecular studies of fishes routinely use tissues preserved in ethanol; yet tissues preserved in this way may yield degraded sequences over time. As an alternative to tissue fixation methods, we assessed and compared previously published cryopreservation methods by gating and counting fish testicular cells with flow cytometry to identify presumptive spermatogonia A-type cells. Here we describe a protocol to cryopreserve tissues that yields a high percentage of viable spermatogonial cells from the testes of Asterropteryx semipunctata, a marine goby. Material cryopreserved using this protocol represents the first frozen and post-thaw viable spermatogonial cells of fishes archived in a natural history museum to provide better quality material for re-derivation of species and DNA preservation and analysis. en
dc.relation.ispartof Scientific Reports en
dc.title Cryopreservation of Fish Spermatogonial Cells: The Future of Natural History Collections en
dc.type Journal Article en
dc.identifier.srbnumber 146242
dc.identifier.doi 10.1038/s41598-018-24269-3
rft.jtitle Scientific Reports
rft.volume 8
rft.spage 6149
rft.epage 6149
dc.description.SIUnit NMNH en
dc.description.SIUnit NH-Vertebrate Zoology en
dc.description.SIUnit NZP en
dc.description.SIUnit Peer-reviewed en
dc.citation.spage 6149
dc.citation.epage 6149

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