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Genomic and transcriptomic resources for assassin flies including the complete genome sequence of <I>Proctacanthus coquilletti</I> (Insecta: Diptera: Asilidae) and 16 representative transcriptomes

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dc.contributor.author Dikow, Rebecca B. en
dc.contributor.author Frandsen, Paul B. en
dc.contributor.author Turcatel, Mauren en
dc.contributor.author Dikow, Torsten en
dc.date.accessioned 2017-02-14T20:56:03Z
dc.date.available 2017-02-14T20:56:03Z
dc.date.issued 2017
dc.identifier.citation Dikow, Rebecca B., Frandsen, Paul B., Turcatel, Mauren, and Dikow, Torsten. 2017. "<a href="https://repository.si.edu/handle/10088/30740">Genomic and transcriptomic resources for assassin flies including the complete genome sequence of Proctacanthus coquilletti (Insecta: Diptera: Asilidae) and 16 representative transcriptomes</a>." <em>PeerJ</em>. 5:e2951. <a href="https://doi.org/10.7717/peerj.2951">https://doi.org/10.7717/peerj.2951</a> en
dc.identifier.issn 2167-8359
dc.identifier.uri https://hdl.handle.net/10088/30740
dc.description.abstract A high-quality draft genome for Proctacanthus coquilletti (Insecta: Diptera: Asilidae) is presented along with transcriptomes for 16 Diptera species from five families: Asilidae, Apioceridae, Bombyliidae, Mydidae, and Tabanidae. Genome sequencing reveals that P. coquilletti has a genome size of approximately 210 Mbp and remarkably low heterozygosity (0.47%) and few repeats (15%). These characteristics helped produce a highly contiguous (N50 = 862 kbp) assembly, particularly given that only a single 2 × 250 bp PCR-free Illumina library was sequenced. A phylogenomic hypothesis is presented based on thousands of putative orthologs across the 16 transcriptomes. Phylogenetic relationships support the sister group relationship of Apioceridae + Mydidae to Asilidae. A time-calibrated phylogeny is also presented, with seven fossil calibration points, which suggests an older age of the split among Apioceridae, Asilidae, and Mydidae (158 mya) and Apioceridae and Mydidae (135 mya) than proposed in the AToL FlyTree project. Future studies will be able to take advantage of the resources presented here in order to produce large scale phylogenomic and evolutionary studies of assassin fly phylogeny, life histories, or venom. The bioinformatics tools and workflow presented here will be useful to others wishing to generate de novo genomic resources in species-rich taxa without a closely-related reference genome. en
dc.relation.ispartof PeerJ en
dc.title Genomic and transcriptomic resources for assassin flies including the complete genome sequence of <I>Proctacanthus coquilletti</I> (Insecta: Diptera: Asilidae) and 16 representative transcriptomes en
dc.type Journal Article en
dc.identifier.srbnumber 141538
dc.identifier.doi 10.7717/peerj.2951
rft.jtitle PeerJ
rft.volume 5
rft.spage e2951
dc.description.SIUnit NH-Entomology en
dc.description.SIUnit NMNH en
dc.description.SIUnit SI-Other en
dc.citation.spage e2951


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