DSpace Repository

An automated monitoring and control system for flow-through co-cycling hypoxia and pH experiments

Show simple item record

dc.contributor.author Burrell, Rebecca B. en
dc.contributor.author Keppel, Andrew G. en
dc.contributor.author Clark, Virginia M. en
dc.contributor.author Breitburg, Denise L. en
dc.date.accessioned 2015-12-23T17:24:19Z
dc.date.available 2015-12-23T17:24:19Z
dc.date.issued 2016
dc.identifier.citation Burrell, Rebecca B., Keppel, Andrew G., Clark, Virginia M., and Breitburg, Denise L. 2016. "<a href="https://repository.si.edu/handle/10088/27765">An automated monitoring and control system for flow-through co-cycling hypoxia and pH experiments</a>." <em>Limnology and Oceanography: Methods</em>. 14 (3):168&ndash;185. <a href="https://doi.org/10.1002/lom3.10077">https://doi.org/10.1002/lom3.10077</a> en
dc.identifier.issn 1541-5856
dc.identifier.uri http://hdl.handle.net/10088/27765
dc.description.abstract Acidification research has exploded in recent years, however, experiments testing effects of co-cycling hypoxia and pH on ecological and physiological processes are rare, despite the pervasiveness and potential importance of co-varying fluctuations in these parameters. Co-cycling dissolved oxygen (DO) and pH are difficult to precisely control, as gases used for manipulation influence both parameters. We successfully developed a LabVIEW(TM)-based system capable of monitoring and controlling co-varying DO and pH in raw seawater flow-through aquaria. Using feedback from Oxyguard DO probes and Honeywell ion sensitive field effect transistor Durafet pH sensors, our system controls ratios of nitrogen, oxygen, carbon dioxide, atmospheric air, and CO2-stripped air within a total gas flow rate through mass flow controllers, to achieve target co-cycling DO and pH values in five treatments. Our system performed well in two long-term experiments investigating effects of diel-cycling hypoxia and pH on eastern oyster (Crassostrea virginica) feeding, growth, fecundity, Perkinsus sp. (Dermo) infection dynamics and immune response. In our 2013 adult oyster experiment, the severe low DO treatment averaged only 0.04 mg L-1 higher than the 0.50 mg L-1 target, and the moderate hypoxia averaged only 0.05 mg L-1 higher than the 1.30 mg L-1 target over 48 d of cycles. Mean pH for the hypercapnia plateau was within 0.02 above the 7.00 target. In our 2013 spat experiment, daily minimum DO in the severe and moderate hypoxia treatments were both within 0.06 mg L-1 of the 0.50 and 1.3 mg L-1 targets, respectively; hypercapnia plateau pH values were within 0.01 of our 7.00 target. en
dc.relation.ispartof Limnology and Oceanography: Methods en
dc.title An automated monitoring and control system for flow-through co-cycling hypoxia and pH experiments en
dc.type Journal Article en
dc.identifier.srbnumber 138229
dc.identifier.doi 10.1002/lom3.10077
rft.jtitle Limnology and Oceanography: Methods
rft.volume 14
rft.issue 3
rft.spage 168
rft.epage 185
dc.description.SIUnit NH-SMS en
dc.description.SIUnit SERC en
dc.description.SIUnit Peer-reviewed en
dc.description.SIUnit NMNH en
dc.citation.spage 168
dc.citation.epage 185

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


My Account