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Mineralization defects in cementum and craniofacial bone from loss of bone sialoprotein

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dc.contributor.author Foster, B. L. en
dc.contributor.author Ao, M. en
dc.contributor.author Willoughby, C. en
dc.contributor.author Soenjaya, Y. en
dc.contributor.author Holm, E. en
dc.contributor.author Lukashova, L. en
dc.contributor.author Tran, A. B. en
dc.contributor.author Wimer, Helen F. en
dc.contributor.author Zerfas, P. M. en
dc.contributor.author Nociti, F. H., Jr. en
dc.contributor.author Kantovitz, K. R. en
dc.contributor.author Quan, B. D. en
dc.contributor.author Sone, E. D. en
dc.contributor.author Goldberg, H. A. en
dc.contributor.author Somerman, M. J. en
dc.date.accessioned 2015-05-19T13:21:10Z
dc.date.available 2015-05-19T13:21:10Z
dc.date.issued 2015
dc.identifier.citation Foster, B. L., Ao, M., Willoughby, C., Soenjaya, Y., Holm, E., Lukashova, L., Tran, A. B., Wimer, Helen F., Zerfas, P. M., Nociti, F. H., Jr., Kantovitz, K. R., Quan, B. D., Sone, E. D., Goldberg, H. A., and Somerman, M. J. 2015. "<a href="https%3A%2F%2Frepository.si.edu%2Fhandle%2F10088%2F26277">Mineralization defects in cementum and craniofacial bone from loss of bone sialoprotein</a>." <em>Bone</em>. 78:150&ndash;164. <a href="https://doi.org/10.1016/j.bone.2015.05.007">https://doi.org/10.1016/j.bone.2015.05.007</a> en
dc.identifier.issn 8756-3282
dc.identifier.uri http://hdl.handle.net/10088/26277
dc.description.abstract Bone sialoprotein (BSP) is a multifunctional extracellular matrix protein found in mineralized tissues, including bone, cartilage, tooth root cementum (both acellular and cellular types), and dentin. In order to define the role BSP plays in the process of biomineralization of these tissues, we analyzed cementogenesis, dentinogenesis, and osteogenesis (intramembranous and endochondral) in craniofacial bone in Bsp null mice and wild-type (WT) controls over a developmental period (1 60 days post natal; dpn) by histology, immunohistochemistry, undecalcified histochemistry, microcomputed tomography (microCT), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and quantitative PCR (qPCR). Regions of intramembranous ossification in the alveolus, mandible, and calvaria presented delayed mineralization and osteoid accumulation, assessed by von Kossa and Goldner s trichrome stains at 1 and 14 dpn. Moreover, Bsp-/- mice featured increased cranial suture size at the early time point, 1 dpn. Immunostaining and PCR demonstrated that osteoblast markers, osterix, alkaline phosphatase, and osteopontin were unchanged in Bsp null mandibles compared to WT. Bsp-/- mouse molars featured a lack of functional acellular cementum formation by histology, SEM, and TEM, and subsequent loss of Sharpey s collagen fiber insertion into the tooth root structure. Bsp-/- mouse alveolar and mandibular bone featured equivalent or fewer osteoclasts at early ages (1 and 14 dpn), however, increased RANKL immunostaining and mRNA, and significantly increased number of osteoclast-like cells (2 5 fold) were found at later ages (26 and 60 dpn), corresponding to periodontal breakdown and severe alveolar bone resorption observed following molar teeth entering occlusion. Dentin formation was unperturbed in Bsp-/- mouse molars, with no delay in mineralization, no alteration in dentin dimensions, and no differences in odontoblast markers analyzed. No defects were identified in endochondral ossification in the cranial base, and craniofacial morphology was unaffected in Bsp-/- mice. These analyses confirm a critical role for BSP in processes of cementogenesis and intramembranous ossification of craniofacial bone, whereas endochondral ossification in the cranial base was minimally affected and dentinogenesis was normal in Bsp-/- molar teeth. Dissimilar effects of loss of BSP on mineralization of dental and craniofacial tissues suggest local differences in the role of BSP and/or yet to be defined interactions with site-specific factors. en
dc.relation.ispartof Bone en
dc.title Mineralization defects in cementum and craniofacial bone from loss of bone sialoprotein en
dc.type Journal Article en
dc.identifier.srbnumber 135967
dc.identifier.doi 10.1016/j.bone.2015.05.007
rft.jtitle Bone
rft.volume 78
rft.spage 150
rft.epage 164
dc.description.SIUnit NH-Vertebrate Zoology en
dc.description.SIUnit NMNH en
dc.description.SIUnit Peer-reviewed en
dc.citation.spage 150
dc.citation.epage 164


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