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Cholesterol addition aids the cryopreservation of dromedary camel (<I>Camelus dromedarius</I>) spermatozoa

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dc.contributor.author Crichton, Elizabeth G. en
dc.contributor.author Pukazhenthi, Budhan S. en
dc.contributor.author Billah, M. en
dc.contributor.author Skidmore, Julian A. en
dc.date.accessioned 2015-04-20T15:16:07Z
dc.date.available 2015-04-20T15:16:07Z
dc.date.issued 2015
dc.identifier.citation Crichton, Elizabeth G., Pukazhenthi, Budhan S., Billah, M., and Skidmore, Julian A. 2015. "Cholesterol addition aids the cryopreservation of dromedary camel (Camelus dromedarius) spermatozoa." <em>Theriogenology</em>. 83 (2):168&ndash;174. <a href="https://doi.org/10.1016/j.theriogenology.2014.09.005">https://doi.org/10.1016/j.theriogenology.2014.09.005</a> en
dc.identifier.issn 0093-691X
dc.identifier.uri http://hdl.handle.net/10088/25825
dc.description.abstract The cryopreservation of dromedary camel (Camelus dromedarius) sperm has proved challenging with little success reported. Yet the routine application of artificial insemination with frozen semen would assist the flow of valuable genetic material nationally and internationally. The current study sought to examine the effects of cholesterol (cholesterol-loaded cyclodextrin; CLC) pre-loading on camel sperm cryosurvival. Ejaculates (n = 3 males; 3 ejaculates/male) were collected using an artificial vagina during the breeding season and extended in Hepes- buffered TALP and allowed to liquefy in the presence of papain ( 0.1 mg/mL) prior to removal of the seminal plasma by centrifugation. Sperm pellets were re-suspended (120 million/mL) in fresh TALP and incubated (15 min; 37 °C) with 0, 1.5 or 4.5 mg CLC/mL. Sperm suspensions were then centrifuged and reconstituted in INRA-96® containing 20% (v:v) egg yolk and 2.5% (v:v) methylformamide, loaded in 0.5 mL plastic straws, sealed and cooled for 20 min at 4°C. Straws were frozen over liquid nitrogen (4 cm above liquid; 15 min), plunged and stored. Sperm motility, forward progressive status and acrosomal integrity were recorded at zero and 3 h post thaw and compared with these same parameters pre-freeze. Aliquots also were stained with chlortetracycline hydrochloride to assess spontaneous sperm capacitation status pre- and post-thaw. Pre-treatment with CLC (1.5 mg/mL and 4.5 mg/mL) enhanced cryosurvival (P 0.05) effect. In summary, dromedary camel sperm benefit from exposure to CLC prior to cryopreservation; this may facilitate the routine collection and storage of sperm from this species. en
dc.relation.ispartof Theriogenology en
dc.title Cholesterol addition aids the cryopreservation of dromedary camel (<I>Camelus dromedarius</I>) spermatozoa en
dc.type Journal Article en
dc.identifier.srbnumber 127970
dc.identifier.doi 10.1016/j.theriogenology.2014.09.005
rft.jtitle Theriogenology
rft.volume 83
rft.issue 2
rft.spage 168
rft.epage 174
dc.description.SIUnit NZP en
dc.description.SIUnit Peer-reviewed en
dc.citation.spage 168
dc.citation.epage 174

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