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FT-Raman spectroscopy as a method for screening collagen diagenesis in bone

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dc.contributor.author France, Christine A. M. en
dc.contributor.author Thomas, Daniel B. en
dc.contributor.author Doney, Charlotte R. en
dc.contributor.author Madden, Odile en
dc.date.accessioned 2015-04-20T15:15:59Z
dc.date.available 2015-04-20T15:15:59Z
dc.date.issued 2014
dc.identifier.citation France, Christine A. M., Thomas, Daniel B., Doney, Charlotte R., and Madden, Odile. 2014. "<a href="https%3A%2F%2Frepository.si.edu%2Fhandle%2F10088%2F25704">FT-Raman spectroscopy as a method for screening collagen diagenesis in bone</a>." <em>Journal of Archaeological Science</em>. 42:346&ndash;355. <a href="https://doi.org/10.1016/j.jas.2013.11.020">https://doi.org/10.1016/j.jas.2013.11.020</a> en
dc.identifier.issn 0305-4403
dc.identifier.uri http://hdl.handle.net/10088/25704
dc.description.abstract Abstract This study examines Fourier transform (FT) Raman spectroscopy as a non-destructive screening method to determine collagen quality in archaeological and paleontological bones. Bone samples were characterized for collagen quality using well-established elemental abundance analyses (i.e., percentage nitrogen and C:N) as the primary criteria for classification. FT-Raman spectra were collected from outer surfaces and freshly cut cross-sections of both well-preserved and poorly-preserved historic mammal bones. Peak heights and peak areas were studied visually and with bivariate and multivariate statistics. Raman spectra from cross-sections provided the most accurate determination of collagen quality. A ratio of the 960 cm-1 and 1636 cm-1 peak heights provided the most unambiguous distinction between bones with well-preserved and poorly-preserved collagen. The 960 cm-1 and 1636 cm-1 peaks represent phosphate anion stretching in the bone mineral and amide carbonyl stretching in the collagen, respectively. FT-Raman spectra from bones with well-preserved collagen produced a 960 cm-1:1636 cm-1 ratio of 19.4 or less (after peaks were baseline corrected). This mineral to collagen ratio was typically greater in poorly-preserved samples as organic material tends to be more susceptible to early stages of diagenesis. These criteria now can be used to accurately determine collagen quality in bones before sacrificing samples to the lengthy and destructive chemical extractions necessary for carbon-14 dating, stable isotope analyses, proteomic analyses, and other techniques of archaeological or paleontological interest. en
dc.relation.ispartof Journal of Archaeological Science en
dc.title FT-Raman spectroscopy as a method for screening collagen diagenesis in bone en
dc.type Journal Article en
dc.identifier.srbnumber 118003
dc.identifier.doi 10.1016/j.jas.2013.11.020
rft.jtitle Journal of Archaeological Science
rft.volume 42
rft.spage 346
rft.epage 355
dc.description.SIUnit MCI en
dc.description.SIUnit NH-Vertebrate Zoology en
dc.description.SIUnit NMNH en
dc.description.SIUnit Peer-reviewed en
dc.citation.spage 346
dc.citation.epage 355


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