dc.contributor.author |
Chen, Juan |
en |
dc.contributor.author |
Zhao, Jietang |
en |
dc.contributor.author |
Erickson, David L. |
en |
dc.contributor.author |
Xia, Nianhe |
en |
dc.contributor.author |
Kress, W. John |
en |
dc.date.accessioned |
2015-04-20T15:15:59Z |
|
dc.date.available |
2015-04-20T15:15:59Z |
|
dc.date.issued |
2015 |
|
dc.identifier.citation |
Chen, Juan, Zhao, Jietang, Erickson, David L., Xia, Nianhe, and Kress, W. John. 2015. "Testing DNA barcodes in closely related species of Curcuma (Zingiberaceae) from Myanmar and China." <em>Molecular Ecology Resources</em>. 15 (2):337–348. <a href="https://doi.org/10.1111/1755-0998.12319">https://doi.org/10.1111/1755-0998.12319</a> |
en |
dc.identifier.issn |
1755-098X |
|
dc.identifier.uri |
http://hdl.handle.net/10088/25699 |
|
dc.description.abstract |
The genus Curcuma L. is commonly used as spices, medicines, dyes and ornamentals. Owing to its economic significance and lack of clear-cut morphological differences between species, this genus is an ideal case for developing DNA barcodes. In the present study, four chloroplast DNA regions (matK, rbcL, trnH-psbA, trnL-F) and one nuclear region (ITS2) were generated for forty-four Curcuma species and five species from closely related genera, represented by 96 samples. PCR amplification success rate, intra- and inter-specific genetic distance variation, and the correct identification percentage were taken into account to assess candidate barcode regions. PCR and sequence success rate were high in matK (89.7%), rbcL (100%), trnH-psbA (100%), trnL-F (95.7%) and ITS2 (82.6%) regions. The results further showed that four candidate chloroplast barcoding regions (matK, rbcL, trnH-psbA and trnL-F) yield no barcode gaps, indicating that the genus Curcuma represents a challenging group for DNA barcoding. The ITS2 region presented large interspecific variation, and provided the highest correct identification rates (46.7%) based on BLASTClust method among the five regions. However, the ITS2 only provided 7.9% based on NJ tree method. An increase in discriminatory power needs the development of more variable markers. This article is protected by copyright. All rights reserved. |
en |
dc.relation.ispartof |
Molecular Ecology Resources |
en |
dc.title |
Testing DNA barcodes in closely related species of <I>Curcuma</I> (Zingiberaceae) from Myanmar and China |
en |
dc.type |
Journal Article |
en |
dc.identifier.srbnumber |
127897 |
|
dc.identifier.doi |
10.1111/1755-0998.12319 |
|
rft.jtitle |
Molecular Ecology Resources |
|
rft.volume |
15 |
|
rft.issue |
2 |
|
rft.spage |
337 |
|
rft.epage |
348 |
|
dc.description.SIUnit |
NH-Botany |
en |
dc.description.SIUnit |
NMNH |
en |
dc.description.SIUnit |
Peer-reviewed |
en |
dc.citation.spage |
337 |
|
dc.citation.epage |
348 |
|