dc.contributor.author |
García-Robledo, Carlos |
en |
dc.contributor.author |
Erickson, David L. |
en |
dc.contributor.author |
Staines, Charles L. |
en |
dc.contributor.author |
Erwin, Terry L. |
en |
dc.contributor.author |
Kress, W. John |
en |
dc.date.accessioned |
2013-09-13T17:14:18Z |
|
dc.date.available |
2013-09-13T17:14:18Z |
|
dc.date.issued |
2013 |
|
dc.identifier.citation |
García-Robledo, Carlos, Erickson, David L., Staines, Charles L., Erwin, Terry L., and Kress, W. John. 2013. "<a href="https://repository.si.edu/handle/10088/21389">Tropical Plant–Herbivore Networks: Reconstructing Species Interactions Using DNA Barcodes</a>." <em>PLoS ONE</em>. 8 (1):e52967. <a href="https://doi.org/10.1371/journal.pone.0052967">https://doi.org/10.1371/journal.pone.0052967</a> |
en |
dc.identifier.issn |
1932-6203 |
|
dc.identifier.uri |
http://hdl.handle.net/10088/21389 |
|
dc.description.abstract |
Plants and their associated insect herbivores, represent more than 50% of all known species on earth. The first step in understanding the mechanisms generating and maintaining this important component of biodiversity is to identify plant-herbivore associations. In this study we determined insect-host plant associations for an entire guild of insect herbivores using plant DNA extracted from insect gut contents. Over two years, in a tropical rain forest in Costa Rica (La Selva Biological Station), we recorded the full diet breadth of rolled-leaf beetles, a group of herbivores that feed on plants in the order Zingiberales. Field observations were used to determine the accuracy of diet identifications using a three-locus DNA barcode (rbcL, trnH-psbA and ITS2). Using extraction techniques for ancient DNA, we obtained high-quality sequences for two of these loci from gut contents (rbcL and ITS2). Sequences were then compared to a comprehensive DNA barcode library of the Zingiberales. The rbcL locus identified host plants to family (success/sequence?=?58.8%) and genus (success/sequence?=?47%). For all Zingiberales except Heliconiaceae, ITS2 successfully identified host plants to genus (success/sequence?=?67.1%) and species (success/sequence?=?61.6%). Kindt s sampling estimates suggest that by collecting ca. four individuals representing each plant-herbivore interaction, 99% of all host associations included in this study can be identified to genus. For plants that amplified ITS2, 99% of the hosts can be identified to species after collecting at least four individuals representing each interaction. Our study demonstrates that host plant identifications at the species-level using DNA barcodes are feasible, cost-effective, and reliable, and that reconstructing plant-herbivore networks with these methods will become the standard for a detailed understanding of these interactions. |
en |
dc.relation.ispartof |
PLoS ONE |
en |
dc.title |
Tropical Plant Herbivore Networks: Reconstructing Species Interactions Using DNA Barcodes |
en |
dc.type |
Journal Article |
en |
dc.identifier.srbnumber |
114230 |
|
dc.identifier.doi |
10.1371/journal.pone.0052967 |
|
rft.jtitle |
PLoS ONE |
|
rft.volume |
8 |
|
rft.issue |
1 |
|
rft.spage |
e52967 |
|
dc.description.SIUnit |
NH-Botany |
en |
dc.description.SIUnit |
NH-Entomology |
en |
dc.description.SIUnit |
NMNH |
en |
dc.description.SIUnit |
Peer-reviewed |
en |
dc.citation.spage |
e52967 |
|