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Identifying gastropod spawn from DNA barcodes: possible but not yet practicable

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dc.contributor.author Puillandre, N. en
dc.contributor.author Strong, Ellen E. en
dc.contributor.author Bouchet, Philippe en
dc.contributor.author Boisselier, M. -C en
dc.contributor.author Couloux, A. en
dc.contributor.author Samadi, S. en
dc.date.accessioned 2012-05-21T19:24:18Z
dc.date.available 2012-05-21T19:24:18Z
dc.date.issued 2009
dc.identifier.citation Puillandre, N., Strong, Ellen E., Bouchet, Philippe, Boisselier, M. -C, Couloux, A., and Samadi, S. 2009. "<a href="https%3A%2F%2Frepository.si.edu%2Fhandle%2F10088%2F18478">Identifying gastropod spawn from DNA barcodes: possible but not yet practicable</a>." <em>Molecular Ecology Resources</em>. 9 (5):1311&ndash;1321. <a href="https://doi.org/10.1111/j.1755-0998.2009.02576.x">https://doi.org/10.1111/j.1755-0998.2009.02576.x</a> en
dc.identifier.issn 1755-098X
dc.identifier.uri http://hdl.handle.net/10088/18478
dc.description.abstract Identifying life stages of species with complex life histories is problematic as species are often only known and/or described from a single stage. DNA barcoding has been touted as an important tool for linking life-history stages of the same species. To test the current efficacy of DNA barcodes for identifying unknown mollusk life stages, 24 marine gastropod egg capsules were collected off the Philippines in deep water and sequenced for partial fragments of the COI, 16S and 12S mitochondrial genes. Two egg capsules of known shallow-water Mediterranean species were used to calibrate the method. These sequences were compared to those available in GenBank and the Barcode of Life Database (BOLD). Using COI sequences alone, only a single Mediterranean egg capsule was identified to species, and a single Philippine egg capsule was identified tentatively to genus; all other COI sequences recovered matches between 76% and 90% with sequences from BOLD and GenBank. Similarity-based identification using all three markers confirmed the Mediterranean specimens&#39; identifications. A phylogenetic approach was also implemented to confirm similarity-based identifications and provide a higher-taxonomic identification when species-level identifications were not possible. Comparison of available GenBank sequences to the diversity curve of a well-sampled coral reef habitat in New Caledonia highlights the poor taxonomic coverage achieved at present in existing genetic databases, emphasizing the need to develop DNA barcoding projects for megadiverse and often taxonomically challenging groups such as mollusks, to fully realize its potential as an identification and discovery tool. en
dc.relation.ispartof Molecular Ecology Resources en
dc.title Identifying gastropod spawn from DNA barcodes: possible but not yet practicable en
dc.type Journal Article en
dc.identifier.srbnumber 79784
dc.identifier.doi 10.1111/j.1755-0998.2009.02576.x
rft.jtitle Molecular Ecology Resources
rft.volume 9
rft.issue 5
rft.spage 1311
rft.epage 1321
dc.description.SIUnit NH-Invertebrate Zoology en
dc.description.SIUnit NMNH en
dc.citation.spage 1311
dc.citation.epage 1321

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