Short-term and long-term effects of temperature on photosynthesis in the diatom <I>Thalassiosira pseudonana </I>under UVR exposure

Abstract

Temperature is expected to modify the effects of ultraviolet radiation (UVR) on photosynthesis by affecting the rate of repair. We studied the effect of short-term (1 h) and long-term (days) acclimation to temperature on UVR photoinhibition in the diatom <I>Thalassiosira pseudonana</I> Hasle et Heimdal. Photosynthesis was measured during 1 h exposures to varying irradiances of PAR and UVR + PAR at 15, 20, and 25°C, the latter corresponding to the upper temperature limit for optimal growth in <I>T. pseudonana</I>. The exposures allowed the estimation of photosynthesis-irradiance (P-E) curves and biological weighting functions (BWFs) for photoinhibition. For the growth conditions used, temperature did not affect photosynthesis under PAR. However, photoinhibition by UVR was highly affected by temperature. For cultures preacclimated to 20°C, the extent of UVR photoinhibition increased with decreasing temperature, from 63% inhibition of PAR-only photosynthesis at 25°C to 71% at 20°C and 85% at 15°C. These effects were slightly modified after several days of acclimation: UVR photoinhibition increased from 63% to 75% at 25°C and decreased from 85% to 80% at 15°C. Time courses of photochemical efficiency (F<SUB>PSII</SUB>) under UVR + PAR were also fitted to a model of UVR photoinhibition, allowing the estimation of the rates of damage (<I>k</I>) and repair (<I>r</I>). The <I>r</I>/<I>k</I> values obtained for each temperature treatment verified the responses observed with the BWF (<I>R</I><SUP>2</SUP> = 0.94). The results demonstrated the relevance of temperature in determining primary productivity under UVR exposures. However, the results suggested that temperature and UVR interact mainly over short (hours) rather than long (days) timescales.