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Soil organic phosphorus in tropical forests: an assessment of the NaOH-EDTA extraction procedure for quantitative analysis by solution 31P NMR spectroscopy

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dc.contributor.author Turner, Benjamin L. en
dc.date.accessioned 2011-02-16T18:27:54Z
dc.date.available 2011-02-16T18:27:54Z
dc.date.issued 2008
dc.identifier.citation Turner, Benjamin L. 2008. "<a href="https%3A%2F%2Frepository.si.edu%2Fhandle%2F10088%2F12209">Soil organic phosphorus in tropical forests: an assessment of the NaOH-EDTA extraction procedure for quantitative analysis by solution 31P NMR spectroscopy</a>." <em>European Journal of Soil Science</em>. 59 (3):453&ndash;466. <a href="https://doi.org/10.1111/j.1365-2389.2007.00994.x">https://doi.org/10.1111/j.1365-2389.2007.00994.x</a> en
dc.identifier.uri http://hdl.handle.net/10088/12209
dc.description.abstract Summary The extraction of soil organic phosphorus by the NaOH-EDTA procedure was assessed in detail for a tropical forest soil (clay-loam, pH 4.3, total carbon 2.7%). Optimum conditions for the quantification of soil organic phosphorus and characterization of its composition by solution 31P NMR spectroscopy were extraction in a solution containing 0.25 m NaOH and 50 mm Na2EDTA in a 1:20 solid to solution ratio for 4 hours at ambient laboratory temperature. Replicate analyses yielded a coefficient of variation of 3% for organic phosphorus as a proportion of the spectral area. There was no significant difference in total phosphorus extraction from fresh and air-dried soil, although slightly more organic phosphorus and less paramagnetic ions were extracted from dried soil. The procedure was not improved by changing the concentration of NaOH or EDTA, extraction time, or solid to solution ratio. Pre-extraction with HCl or Na2EDTA did not increase subsequent organic phosphorus extraction in NaOH-EDTA or improve spectral resolution in solution 31P NMR spectroscopy. Post-extraction treatment with Chelex resin did not improve spectral resolution, but removed small concentrations of phosphorus from the extracts. Increasing the pH of NaOH-EDTA extracts (up to 1.0 m NaOH) increased the concentration of phosphate monoesters, but decreased DNA to an undetectable level, indicating its hydrolysis in strong alkali. The standardized NaOH-EDTA extraction procedure is therefore recommended for the analysis of organic phosphorus in tropical forest soils. en
dc.relation.ispartof European Journal of Soil Science en
dc.title Soil organic phosphorus in tropical forests: an assessment of the NaOH-EDTA extraction procedure for quantitative analysis by solution 31P NMR spectroscopy en
dc.type Journal Article en
dc.identifier.srbnumber 55697
dc.identifier.doi 10.1111/j.1365-2389.2007.00994.x
rft.jtitle European Journal of Soil Science
rft.volume 59
rft.issue 3
rft.spage 453
rft.epage 466
dc.description.SIUnit Encyclopedia of Life en
dc.description.SIUnit Forces of Change en
dc.description.SIUnit STRI en
dc.citation.spage 453
dc.citation.epage 466


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