dc.contributor.author |
Basset, Yves |
en |
dc.contributor.author |
Donoso, David A. |
en |
dc.contributor.author |
Hajibabaei, Mehrdad |
en |
dc.contributor.author |
Wright, Michael T. G. |
en |
dc.contributor.author |
Perez, Kate H. J. |
en |
dc.contributor.author |
Lamarre, Greg P. A. |
en |
dc.contributor.author |
De León, Luis F. |
en |
dc.contributor.author |
Palacios-Vargas, Jos |
en |
dc.contributor.author |
Castaño-Meneses, Gabriela |
en |
dc.contributor.author |
Rivera, Marleny |
en |
dc.contributor.author |
Perez, Filonila |
en |
dc.contributor.author |
Bobadilla, Ricardo |
en |
dc.contributor.author |
Lopez, Yacksecari |
en |
dc.contributor.author |
Ramirez, José Alejandro |
en |
dc.contributor.author |
Barrios, Héctor |
en |
dc.date.accessioned |
2021-12-07T02:32:24Z |
|
dc.date.available |
2021-12-07T02:32:24Z |
|
dc.date.issued |
2020 |
|
dc.identifier.citation |
Basset, Yves, Donoso, David A., Hajibabaei, Mehrdad, Wright, Michael T. G., Perez, Kate H. J., Lamarre, Greg P. A., De León, Luis F., Palacios-Vargas, Jos, Castaño-Meneses, Gabriela, Rivera, Marleny, Perez, Filonila, Bobadilla, Ricardo, Lopez, Yacksecari, Ramirez, José Alejandro, and Barrios, Héctor. 2020. "<a href="https://mbmg.pensoft.net/article/58572/; https://mbmg.pensoft.net/article/58572/download/pdf/">Methodological considerations for monitoring soil/litter arthropods in tropical rainforests using DNA metabarcoding, with a special emphasis on ants, springtails and termites</a>." <em>Metabarcoding and Metagenomics</em> 4:<a href="https://doi.org/10.3897/mbmg.4.58572">https://doi.org/10.3897/mbmg.4.58572</a> |
en |
dc.identifier.issn |
2534-9708 |
|
dc.identifier.uri |
https://hdl.handle.net/10088/111912 |
|
dc.description.abstract |
Robust data to refute or support claims of global insect decline are currently lacking, particularly for the soil fauna in the tropics. DNA metabarcoding represents a powerful approach for rigorous spatial and temporal monitoring of the taxonomically challenging soil fauna. Here, we provide a detailed field protocol, which was successfully applied in Barro Colorado Island (BCI) in Panama, to collect soil samples and arthropods in a tropical rainforest, to be later processed with metabarcoding. We also estimate the proportion of soil/litter ant, springtail and termite species from the local fauna that can be detected by metabarcoding samples obtained either from Berlese-Tullgren (soil samples), Malaise or light traps. Each collecting method detected a rather distinct fauna. Soil and Malaise trap samples detected 213 species (73%) of all target species. Malaise trap samples detected many ant species, whereas soil samples were more efficient at detecting springtail and termite species. With respect to long-term monitoring of soil-dwelling and common species (more amenable to statistical trends), the best combination of two methods were soil and light trap samples, detecting 94% of the total of common species. A protocol including 100 soil, 40 Malaise and 80 light trap samples annually processed by metabarcoding would allow the long-term monitoring of at least 11%, 18% and 16% of species of soil/litter ants, springtails and termites, respectively, present on BCI, and a high proportion of the total abundance (up to 80% of all individuals) represented by these taxa. |
en |
dc.relation.ispartof |
Metabarcoding and Metagenomics |
en |
dc.title |
Methodological considerations for monitoring soil/litter arthropods in tropical rainforests using DNA metabarcoding, with a special emphasis on ants, springtails and termites |
en |
dc.type |
Journal Article |
en |
dc.identifier.srbnumber |
161199 |
|
dc.identifier.doi |
10.3897/mbmg.4.58572 |
|
rft.jtitle |
Metabarcoding and Metagenomics |
|
rft.volume |
4 |
|
dc.description.SIUnit |
STRI |
en |
dc.relation.url |
https://mbmg.pensoft.net/article/58572/; https://mbmg.pensoft.net/article/58572/download/pdf/ |
|