dc.contributor.author |
Song, Jiakun |
en |
dc.contributor.author |
Parenti, Lynne R. |
en |
dc.date.accessioned |
2010-08-25T20:39:30Z |
|
dc.date.available |
2010-08-25T20:39:30Z |
|
dc.date.issued |
1995 |
|
dc.identifier.citation |
Song, Jiakun and Parenti, Lynne R. 1995. "<a href="https://repository.si.edu/handle/10088/10955">Clearing and Staining Whole Fish Specimens for Simultaneous Demonstration of Bone, Cartilage, and Nerves</a>." <em>Copeia</em>. 114–118. |
en |
dc.identifier.issn |
0045-8511 |
|
dc.identifier.uri |
http://hdl.handle.net/10088/10955 |
|
dc.description.abstract |
Comparative anatomical studies of nerves alone, or combined with those of bone and cartilage, are rare in ichthyology because of lack of a reliable, repeatable protocol for preparing specimens that demonstrate all three character systems. We present a method for enzyme clearing and staining whole fish specimens for simultaneous demonstration of bone, cartilage, and nerves. Nerves are stained dark blue with Sudan Black B following alcian blue-alizarin red S staining. Our protocol for Sudan Black B staining differs from others in concentration of Sudan Black B solution, length of time in that solution, and method of differentiation. |
en |
dc.relation.ispartof |
Copeia |
en |
dc.title |
Clearing and Staining Whole Fish Specimens for Simultaneous Demonstration of Bone, Cartilage, and Nerves |
en |
dc.type |
Journal Article |
en |
dc.identifier.srbnumber |
74654 |
|
rft.jtitle |
Copeia |
|
rft.issue |
1 |
|
rft.spage |
114 |
|
rft.epage |
118 |
|
dc.description.SIUnit |
NMNH |
en |
dc.description.SIUnit |
NH-Vertebrate Zoology |
en |
dc.citation.spage |
114 |
|
dc.citation.epage |
118 |
|