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Knots, spoons, and cloches: DNA barcoding unusual larval forms helps document the diversity of Neotropical marine annelids

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dc.contributor.author Collin, Rachel en
dc.contributor.author Venera-Ponton, Dagoberto E. en
dc.contributor.author Macdonald, Kenneth en
dc.contributor.author Driskell, Amy C. en
dc.contributor.author Boyle, Michael J. en
dc.date.accessioned 2021-02-26T03:02:53Z
dc.date.available 2021-02-26T03:02:53Z
dc.date.issued 2021
dc.identifier.citation Collin, Rachel, Venera-Ponton, Dagoberto E., Macdonald, Kenneth, Driskell, Amy C., and Boyle, Michael J. 2021. "Knots, spoons, and cloches: DNA barcoding unusual larval forms helps document the diversity of Neotropical marine annelids." <em>Invertebrate Biology</em>. e12311&ndash;e12311. <a href="https://doi.org/10.1111/ivb.12311">https://doi.org/10.1111/ivb.12311</a> en
dc.identifier.issn 1077-8306
dc.identifier.uri https://hdl.handle.net/10088/109384
dc.description.abstract The morphological diversity of marine annelid larvae is stunning. Although many of the larval forms have been categorized as trochophores or modified trochophores, there are a few groups with distinctive larval features that make them easy to distinguish from other annelid larvae. We collected 252 annelid larvae from the plankton, with particular emphasis on oweniids, polygordiids, and thalassematids (i.e., echiurans) and sequenced fragments of their cytochrome c oxidase subunit 1 and 16S ribosomal RNA genes. We found six oweniid, five polygordiid, and eight thalassematid OTUs. Thalassematids were found only in samples from the Pacific, and oweniids were found only in Caribbean samples. Among the oweniids we found two distinct morphotypes, one with a narrow, cloche shape and another that had a wider and more rectangular shape with clearly developed lappets. Among the polygordiids, we identified one larva as Polygordius eschaturus and several larvae as Polygordius jenniferae. All larvae, except for the P. eschaturus, which was at a stage too early to make a determination, were endolarvae. Among the thalassematids, we identified larvae of Ochetostoma edax and found seven unidentified OTUs. Finally, 150 miscellaneous polychaete larvae were sequenced, representing similar to 76 OTUs. Four rostraria larvae from the Caribbean, whose sequences confirm the long-held assumption that they are amphinomids, could not be identified to species. In total only 5% of these OTUs could be identified to species with known sequences, and most could not be identified to genus or even family with reasonable certainty. It is clear that this poor coverage in the reference databases will limit metabarcoding efforts to document numbers of OTUs, and that DNA barcodes will be of limited use for identifying neotropical marine annelids until reference databases have improved their coverage of this group. en
dc.relation.ispartof Invertebrate Biology en
dc.title Knots, spoons, and cloches: DNA barcoding unusual larval forms helps document the diversity of Neotropical marine annelids en
dc.type Journal Article en
dc.identifier.srbnumber 158472
dc.identifier.doi 10.1111/ivb.12311
rft.jtitle Invertebrate Biology
rft.spage e12311
rft.epage e12311
dc.description.SIUnit NMNH en
dc.description.SIUnit NH-SMS en
dc.description.SIUnit STRI en
dc.description.SIUnit NH-Other en
dc.citation.spage e12311
dc.citation.epage e12311

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