DSpace Repository

DNA barcoding of echinopluteus larvae uncovers cryptic diversity in neotropical echinoids

Show simple item record

dc.contributor.author Collin, Rachel en
dc.contributor.author Venera-Ponton, Dagoberto E. en
dc.contributor.author Driskell, Amy C. en
dc.contributor.author Macdonald, Kenneth S. en
dc.contributor.author Geyer, Laura B. en
dc.contributor.author Lessios, Harilaos A. en
dc.contributor.author Boyle, Michael J. en
dc.date.accessioned 2020-06-30T13:44:24Z
dc.date.available 2020-06-30T13:44:24Z
dc.date.issued 2020
dc.identifier.citation Collin, Rachel, Venera-Ponton, Dagoberto E., Driskell, Amy C., Macdonald, Kenneth S., Geyer, Laura B., Lessios, Harilaos A., and Boyle, Michael J. 2020. "<a href="https://repository.si.edu/handle/10088/106142">DNA barcoding of echinopluteus larvae uncovers cryptic diversity in neotropical echinoids</a>." <em>Invertebrate Biology</em>. Article e12292. <a href="https://doi.org/10.1111/ivb.12292">https://doi.org/10.1111/ivb.12292</a> en
dc.identifier.issn 1077-8306
dc.identifier.uri https://hdl.handle.net/10088/106142
dc.description.abstract Surveys of larval diversity consistently increase biodiversity estimates when applied to poorly documented groups of marine invertebrates such as phoronids and hemichordates. However, it remains to be seen how helpful this approach is for detecting unsampled species in well-studied groups. Echinoids represent a large, robust, well-studied macrofauna, with low diversity and low incidence of cryptic species, making them an ideal test case for the efficacy of larval barcoding to discover diversity in such groups. We developed a reference dataset of DNA barcodes for the shallow-water adult echinoids from both coasts of Panama and compared them to DNA sequences obtained from larvae collected primarily on the Caribbean coast of Panama. We sequenced mitochondrialcytochrome c oxidase subunit I(COI) for 43 species of adult sea urchins to expand the number and coverage of sequences available in GenBank. Sequences were successfully obtained forCOIand16Sribosomal DNA from 272 larvae and assigned to 17 operational taxonomic units (OTUs): 4 from the Pacific coast of Panama, where larvae were not sampled as intensively, and 13 from the Caribbean coast. Of these 17 OTUs, 13 were identified from comparisons with our adult sequences and belonged to species well documented in these regions. Another larva was identified from comparisons with unpublished sequences in the Barcode of Life Database (BOLD) as belonging toPseudoboletia, a genus scarcely known in the Caribbean and previously unreported in Panama. Three OTUs remained unidentified. Based on larval morphology, at least two of these OTUs appeared to be spatangoids, which are difficult to collect and whose presence often goes undetected in standard surveys of benthic diversity. Despite its ability to capture unanticipated diversity, larval sampling failed to collect some species that are locally common along the Caribbean coast of Panama, such asLeodia sexiesperforata,Diadema antillarum, andClypeaster rosaceus. en
dc.relation.ispartof Invertebrate Biology en
dc.title DNA barcoding of echinopluteus larvae uncovers cryptic diversity in neotropical echinoids en
dc.type Journal Article en
dc.identifier.srbnumber 155887
dc.identifier.doi 10.1111/ivb.12292
rft.jtitle Invertebrate Biology
rft.spage Article e12292
dc.description.SIUnit STRI en
dc.description.SIUnit NMNH en
dc.description.SIUnit NH-Other en
dc.description.SIUnit NH-SMS en
dc.citation.spage Article e12292

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


My Account