THE NAUTILUS 121(3):146-158, 2007 Page 146 Two new modern records of the southern oyster drill Stranionita haemastoraa floridana (Conrad, 1837) in Chesapeake Bay, USA Juliana M. Harding Department of Fisheries Science Virginia Institute of Marine Science Gloucester Point VA, 23062 USA jharding@\ims.edu M. G. Harasewych Department of Invertebrate Zoology, MRC-163 National Museum of Natural History' Smithsonian Institution, P.O. Box 37012, Washington, DC 20013-7012 USA IIarasewych@si.edu ABSTRACT \A\e southern oyster drills, Stranionita haemastoma floridana (Conrad, 1837), have been collected from two Chesapeake Bay western shore tributaries. Four specimens were collected be- tween Brown Shoal and Thomas Rock in the lower James River in February 2005. Thirteen live southern oyster drills were collected from Back Biver in April 2006. Identification of these drills as Stramonita haemastoma floridana has been confirmed using DNA bar-coding data. Southern oyster drills collected in Chesapeake Bay are genetically nearly identical to populations from the Atlantic coast, and differ significantly from popula- tions from the Azores and from the Gulf of Mexico. These collections mark the first reported records of live southern oys- ter drills from within the Chesapeake Bay estuar)'. It is un- known if these drills represent isolated introductions or expan- sions of the northern range this species. Water temperature patterns in Chesapeake Bay and the Mid-Atlantic Bight from 1990-2005 are similar to those obser\'ed in the late 1950s when Stramonita haemastoma floridana was first found living in Chincoteague Bay, Maryland. Additional Keywords: Bange extension, water temperature. Cape Hatteras, zoogeography, DNA bar-coding INTRODUCTION The southern oyster drill Stramonita haemastoma flori- dana (Conrad, 1837) is a predatory gastropod typically found in association with populations of the oyster Cras- sostrea virginica Gmelin, 1791 (e.g., Burkenroad, 1931; Butler, 1985). A population from the Atlantic coast of the United States was described as Purpura floridana Con- rad, 1837, but later regarded to be a subspecies of the '^ Author for correspondence. broadly ranging Stramonita haemastoma (Linnaeus, 1767), a morphologically diverse taxon that has been re- ported to span the temperate and tropical coasts of the eastern and western Atlantic as well the eastern Pacific. The name Stramonita haemastoma haemastoma (type locality: Tenerife, Canary Islands, fixed by Clench, 1947: 76) has been applied to the populations ranging from the Channel Islands southward to Senegal, the western Mediterranean, the Madeira, Canary and Cape Verde Islands in the eastern Atlantic (Clench, 1947: 76; Poppe and Coto, 1991: 141), the Azores (Morton et al., 1998), Trinidad southward to Uruguay in the western Atlantic (Clench, 1947: 76), and from Baja California southward to Peru in the eastern Pacific by some (e.g.. Clench, 1947) but not all (e.g.. Keen, 1971: 549) researchers. Stramonita haemastoma floridana (type locality: Hy- poluxo Island, Lantana, Florida, see Clench, 1947: 77) was reported to range from North Carolina southward to Yucatan and the West Indies, and throughout the Ca- ribbean as far south as Trinidad (Clench, 1947: 76), or extending to Brazil (Abbott, 1974: 180). The name Stra- monita haemastoma canaliculata (Cray, 1839) (type lo- cality originally but erroneously listed as China) has been applied to a distinctive morphological variant prevalent throughout the Culf of Mexico (e.g., Abbott, 1974: 180). Some three dozen names have been variously partitioned and synonymized for the many geographically circum- scribed shell phenotypes belonging to the Stramonita haemastoma complex (see Rosenberg, 2005). North American records of Stramonita have generally been divided into two subspecies, Stramonita haema- stoma floridana from the Atlantic coastline, and Stra- monita haemastoma canaliculata from the Culf of Mexico; such diwsion is based on shell characters (Ab- bott, 1974; Butler, 1985). Stramonita haemastoma canaliculata has been differentiated morphologically on J. M. Harding and M. G. Harasewych, 2007 Page 147 Figures 1-9. Stramonita spp. 1-6. Stramonita haemastoma floridana (Conrad, 1837). 1. USNM 618840, Willis Wharf, Hog Island Bay, Northumberland Co., Virginia, trapped in 2.4 m depth. September 15, 1955, ex-F. W. Sieling. 2. USNM 1091020, oyster reefs just below Deep Creek, Lower James River, Virginia, in approximately 3 m, Februar)' 2005. 3. Male and 4. female specimen, USNM 1091021, Back Ri\'er, Virginia, in commercial crab pots set in 3 m, April 2006. 5. USNM 416874, Fort Macon, North Carolina. 6. USNM 1099258, Sand bar on north side of Fort Pierce Inlet, E of Little Jim Island, Ft. Pierce, Florida. 6 August 2004. 7. Stramonita canaliculata (Gray, 1839), USNM 1099259, St. Andrews Bay, Florida, rip-rap near campsites. 8. Stramonita haemastoma haysae (Clench, 1927), USNM 568834, Barataria Bay, Louisiana. 9. Stramonita haemastoma haemastoma (Linnaeus, 1767), USNM 1099260, intertidal rocks, Vila Franca do Campo, Sao Miguel, Azores, 26 July 2006. Page 148 THE NAUTILUS, Vol. 121, No. 3 Figure 10. Map oi the Atlantic Ocean depicting the southeastern coast of the United States, Chesapeake Bay region [inset], and the Azores Islands, showing collection sites for Stramonita specimens and water-temperature data. Sites are identified as follows: Delaware lightship and buoy stations (DE), Chincoteague, VA (Al), Chesapeake lightship and buoy stations (C), Fort Pierce Inlet, FL (A2), St. Andrew's Bay, FL (A.3), Pensacola, FL (A4), and Sao Miguel, Azores (A5). Within the Chesapeake Bay region Chincoteague, VA (Bl), Maryland DNB monitoring station XBM1849 (B2), Maryland DNR monitoring station XBM 1.301 (B3), Virginia Institute of Marine Science, Gloucester Point, VA (B4), Back Ri\'er, VA (B5), James River near Deep Creek (B6). the basis of its larger size, presence of strong, rugose shoulder nodules, and its deeply channeled suture. Sev- eral authors (e.g., Butler, 1954; Gunter, 1979; Walker, 1982) had reported that differences in shell morphology are neither consistent nor concordant with geographical patterns and concluded that subspecific distinction was not warranted. More recently, Liu et al. (1991) con- firmed that characters of shell and radula were not taxo- nomically informative, but demonstrated that Stramonita from the northern Gulf of Mexico could be differentiated from populations of the Atlantic coast at "a level that is characteristic of congeneric species" using allozyme elec- trophoresis. Other recent studies have explored the ef- fectiveness of the east Florida ecotone as a barrier to gene flow between Atlantic and Gulf coast populations for a variety of taxa (for reviews, see A\dse, 2000; Wise et al., 2004). Vermeij (2001: 701) reviewed the Recent and fossil species of Stramonita, and suggested that the Stra- J. M. Harding and M. G. Harasewych, 2007 Page 149 Table 1. Samples of Stramonita spp. used in tliis study. Taxon Locality Voucher specimens USNM Number of specimens sequenced GenBank accession number Stramonita canaliculata^^' Stramonita canaliculata Stramonita haem.astom,a haemastoma Stramonita haeniastom,a floridana Stramonita haemastoma floridana Stramonita haemastom,a floridana Pensacola, FL 888709 1 U86330 St. Andrew's Bay, FL 1099259 1 EU073061 Sao Miguel, Azores 1099260 2 EU073051-EU073052 Ft. Fierce Inlet, FL 1099258 2 EU73053-EU073054 Deep Creek, VA 1091020 3 EU073055-EU073057 Back Biver, VA 1091021 3 EU073058-EU073060 (1) Sequence data from Harasewych et al., 1997 m?nita haemastoma complex may consist of as many as four western Atlantic and two eastern Atlantic species. He also noted similarities (bifid crenulations along the outer lip) between some specimens of S. canaliculata and S. bifida Vermeij, 2001, from the Cantaure Formation (early Miocene, Venezuela). Although the northern limit of Stramonita along the eastern United States has generally been reported as Or- egon Inlet, North Carolina (Wells and Grey, 1960; But- ler, 1985), living indiwduals (Figure 1) and recent his- torical specimens (empty shells) were collected from lo- cations in the Maryland and Virginia waters of Ghincoteague Bay (Figure 10, Al) in 1955 and 1956 (Sieling, 1955; 1960), extending the northern range boundary for this animal along the US Atlantic coast. Sieling (1960) suggested that the southern oyster drills were introduced into Ghincoteague Bay with trans- planted oysters. Subsequent surveys of Assateague Island and Ghincoteague Bay by Gounts and Bashore (1991), Homer et al. (1997), and Prezant et al. (2002) reported li\dng S. haemastoma floridana from these habitats as recently as 1996 (Prezant et al., 2002). Gounts and Bashore (1991) categorized S. haemastoma floridana as "rare" and linked the relative decline in abundance and distribution of this drill with the decline in local oyster resources from 1960 to 1989. Prezant et al. (2002) report li\dng S. haemastoma floridana in their survey and col- lected specimen(s) from Memorial Park, Maryland. Liwng species of Stramonita have not previously been reported from the interior of the Ghesapeake Bay (An- drews, 1956; Wass, 1972) in modern time, although S. h. canaliculatum had occurred in Ghesapeake Bay, Mary- land, and S. h. floridana had reached southern New Jer- sey during the exceptionally warm Sangamonian Stage (80,000 to ca. 220,000 yr BP) of the Pleistocene (Petuch 1997: 53). Buiz et al. (2000) reported S. haemastoma as an established resident in the Ghesapeake Bay region on the basis of the Ghincoteague Bay, Maryland-Virginia, Table 2. Summary of water temperature data sources from 1950-2005 for Delaware (DE, Figure 10) and Chesapeake (C, Figure 10). Sea surface temperature (SST), bottom temperature (BT) and air temperature at the water surface (AT) were used. Station Year(s) Data Station type Depth Source DelawareAVinter Quarter Chesapeake 1955-60 SST, BT Winter Quarter lightship 24-29 m 1 1961-70 SST, BT Delaware lightship 30 m 2 1970-84 SST One degree quadrangles NA 3. 1984-2005 AT, SST NOAA buoy 44009 28 m 6 1950-1971 SST Chesapeake lightship 20 m 7. 1971-84 SST One degree quandrangles NA 3. 1985-2005 AT, SST NOAA buoy CHLV2 11.6 m 9 4,5 4,5 1. Winter Quater lightship data archi\'e. East Coast USCG lightship/Lightstations. MBLWHOI Library data archi\'es, Woods Hole, MA. http://dlaweb.whoi.edu/lightshipdightships_winterqtr.html 2. Delaware lightship data archive. East Coast USCG Lightship/Lightstations. MBLWHOI Librar)' data archives. Woods Hole, MA. http://dlaweb.whoi.edu/lightship/lightships_delaware_html 3. 1966-1974. The Gulf Stream. U.S. Na\'al Oc?anographie Office, Vols 1-9. 4. 1975-1980. gulfstream. U.S. Dept. of Commerce, NOAA, National Weather Ser\ice. Vols. 1-6. 5. 1981-94. Oc?anographie Monthly Summary. U.S. Dept. of Commerce, NOAA, National Weather Service/National Earth Satellite Service. Vols. 1-14. 6. Delaware Bay, Buoy 44009. http://www.nodc.noaa.gov/BUOY/44009.html. 7. Bumpus, D. 1957. Surface water temperatures along Atlantic and Gulf coasts of the United States. U.S. Fish and Wildlife Ser\ice Special Scientific Beport?Fisheries No. 214. 8. Chesapeake lightship data archive. East Coast USCG Lightship/Lightstations. MBLWHOI Library data archives. Woods Hole, MA. http://dlaweb.whoi.edu/lightship/lightships_chespstn.html 9. Chesapeake Light, VA, Buoy CHLV2. http://www.nodc.noaa.gov/BUOY/chlv2.html. Page 150 THE NAUTILUS, Vol. 121, No. 3 H?\?\?I?\?\?\?h D 2005: Jamgs River, VA ? 2009: Back ftiver, VA H?\?\?\?h lU -F-H?\?h 64 55 58 70 TZ 74 76 76 60 82 84 Midpolni of shell length class ^mm) 1 \ h Figure 11. Length frequency distribution of live Stramonita haemastoma floridana specimens collected from Back River, VA (2006) and the James River, VA near Deep Creek (2005). and Hog Island Bay, Virginia, collections reported by Sieling (1960) (P. Fofonoff, Smithsonian Environmental Research Center, personal communication). Distribution patterns of many moUuscan species along the US Atlantic coast are directly related to water temperature with Cape Hatteras acting as a natural Zoogeographie boundary (Franz and Merrill, 1980a, b). Wells and Gray (1960) reported S. h. floridana on two subtidal shipwrecks south of Cape Hatteras, North Carolina, but found none on the four shipwrecks they examined north of Cape Hatteras. Wells (1961) described regular collections of resident S. h. floridana from intertidal oyster reefs in the wcinity of Beaufort and Cape Lookout, North Carolina. Southern oyster drills begin laying eggs at water tem- peratures between 20 and 30?C (Stickle, 1999). At water temperatures less than 10-12.5?C southern oyster drills stop feeding on bivalves, bury into the substrate, and become inactive (Bulter, 1954; Gunter, 1979; Garton and Stickle, 1980; Stickle, 1999) until water temperatures rises. While the upper lethal thermal limit for southern oyster drills is 35 to 40? C (Brown and Stickle, 2002), the lower lethal thermal limit for southern oyster drills is unknown. This report documents the first collections of liwng Stramonita haemastoma floridana from within the Chesapeake Bay estuary. We characterize both groups of southern oyster drills collected in the Chesapeake Bay genetically (cytochrome c oxidase I "DNA bar-coding") and compare these data to sequences derived from rep- resentative specimens from the southeastern (Indian River Inlet) and northwestern (St. Andrew's Bay, Pensa- cola) coasts of Florida, as well as from samples from Sao Miguel, Azores. The presence of these drills in Chesa- peake estuarine habitats is analyzed in the context of a long term (1950-2005) coastal bottom water tempera- ture data set and two independently collected estuarine bottom water temperature data sets (Chincoteague Bay, Maryland and Virginia, 1951-56; McGary and Selling, 1953; Selling, 1957; York River, Virginia, 1986-2005; VIMS, 2006) in order to relate the observed Chesapeake collections with environmental/climate conditions in known drill habitats along the Atlantic coast and in Chin- coteague Bay. MATERIALS AND METHODS Sample Collection: Four li\dng S. haemastoma (Fig- ure 2) were collected in the lower James River, Virginia, in the Chesapeake Bay from oyster {Crassostrea vir- ginica) reefs just below Deep Creek (Figure 10, B6) at a depth of approximately 3 m in February 2005 and were turned in to the Virginia Institute of Marine Science (VIMS) rapa whelk [Rapana venosa (Valenciennes, 1846)] bounty program (see Harding and Mann, 2005, for bounty program details). All four Stramonita speci- Table 3. Nucleotide (above diagonal) and amino acid (below diagonal) differences between samples in the portion of the cytochrome c oxidase I gene (591 aligned positions, corresponding to positions 73 to 664 in the complete mitochondrial genome of llyanassa obsoleta [Genbank NC 007781]). Sample 10 11 12 1) S. 2) S. 3) s. 4) s. 5) s. 6) s. 7) s. 8) s. 9) s. 10) s. 11) s. 12) s. canaliculata Pensacola, FL canaliculata St. Andrew's, FL h. h aemastoma Azores 1 h. haemastoma Azores 2 h. floridana Ft. Fierce Inlet, FLl h. floridana Ft. Fierce Inlet, FL2 h. floridana Deep Creek, VAl h. floridana Deep Creek, VA2 h. floridana Deep Creek, VA3 h. floridana Black Ri\'er, VAl h. floridana Black Ri\'er, VA2 h. floridana Black River, VA3 ? 0 126 126 123 123 123 122 124 124 123 122 0 ? 126 126 123 123 123 122 124 124 123 122 8 8 ? 5 56 55 55 58 56 56 56 56 7 n 1 ? 57 56 56 61 57 57 57 57 7 n 3 2 ? 2 2 7 3 5 3 3 7 n 3 2 0 ? 0 5 1 3 1 1 7 y 3 2 0 0 ? 5 1 3 1 1 8 8 2 3 1 1 1 ? 6 8 6 6 7 y 3 2 0 0 0 1 ? 4 2 2 7 n 3 2 0 0 0 1 0 ? 4 4 7 y 3 2 0 0 0 1 0 0 ? 2 7 y 3 2 0 0 0 1 0 0 0 ? J. M. Harding and M. G. Harasewych, 2007 Page 151 -2? S. cana?culata PenFL ?? S. cana?culata SAnFL 38 92 100 100 100 wo 30 100 100 51 J S. haemastom? haemastowa Azores 1 j S. haemastoma haewastoma Azores 2 S. haemastoma floridana FPIFL1 _o S. haemastoma fiondana FP1FL2 ? S. liaemastoma floridana DCVA1 J S. haemastoma floridana DCVA2 J S. haemastoma tloridana DCVA3 J S. haemastoma floridana BRVA1 J S. haemastoma floridana BRVA2 J S. haemastoma floridana BRVA3 Gulf of Mexico Azores Fort Pierce Inlet, FL Deep Creek, VA Back River, VA Figure 12. Strict consensus of four most parsimonious trees (1 = 176; ci = 0.955; ri = 0.960) resulting from an exhaustive search using maximum parsimony (PAUP 4.0bl0) based on 591 bp of cytochrome c oxidase I sequences. Branch lengths from one of the four most parsimonious trees are above the branches, bootstrap (bold) and jackknife (bold italic) propor- tions are below the branches. mens were collected in a single oyster dredge tow. Thir- teen li\ing S. haemastoma (Figures 3^) were collected in Back River, Virginia, (Figure 10, B5) in April 2006 and were also turned into VIMS \da the rapa whelk bounty program. The 2006 collection was made using commer- cial crab pots (wire mesh cubes with approximately 0.5 m sides) baited with northern quahogs [Mercenaria merce- naria (Linnaeus, 1758)] deployed at a depth of 3 m. Shell lengths of specimens (mm, maximum dimension tip of the spire to bottom of the siphonal canal) were measured upon receipt at VIMS. Voucher specimens from both Chesapeake collections have been deposited in the Na- tional Museum of Natural History, Smithsonian Institu- tion (USNM, Table 1). DNA Bar-coding: Three specimens from each Chesa- peake collection were frozen and transported to the labo- ratory, where DNA was extracted from red buccal muscles of each animal using Qiagen DNEasy kits ac- cording to the manufacturer's protocol. A portion of the mitochondrial gene for the DNA bar-coding region of the cytochrome c oxidase I gene was amplified using Sigma JumpStart RedTaq ReadyMix and Meyer's (2003) degenerated versions of the Folmer et al. (1994) HCO and LCO primers for the samples listed in Table 1. The resulting PCR products were cleaned using magnetic beads [Agencourt, manufacturer's protocol] and se- quencing reactions were run on ABI 3730 sequencers that were set up according to manufacturer's instruc- tions. Sequences were manually checked/corrected and assembled using Sequencer^M 4.1 (Gene Codes Corp.), then aligned with Clustal W (Higgins et al., 1994) using default settings, and their relationships analyzed using PAUP 4.0bl0 (Swofford, 2002). Pairwise comparisons of sequences and amino acids were performed using Mega 3.1 (Kumar et al., 2004). Water Temperature Data: COASTAL HABITATS: Wa- ter temperature data from 1950-2005 were used to de- scribe mean monthly bottom water temperatures (BT, C?) for two stations in the Mid-Atlantic Bight (Delaware/ Winter Quarter and Chesapeake) (Figure 10, Table 2). Daily sea surface and bottom water temperature data from the DelawareAVinter Quarter (1961-70) and Chesapeake (1958-71) lightship stations were used to calculate the average monthly observed difference be- tween sea surface temperature (SST) and bottom water temperature (BT) on a site-speci?c basis. The observed average monthly SST-BT differences from the daily lightship data were used to estimate average monthly BT at these sites during years after 1971 when only SST was available (Table 2). Hourly air temperature (AT) and SST data from NOAA buoys (1985-2005) were used to calculate the obsen ed difference between average monthly AT and average monthly SST for Delaware Light and Chesa- peake Light. The obsen ed average monthly AT-SST dif- ferences from the buoy data were used to estimate hourly SST for these sites during months after 1984 when only AT was available. Average residuals for annual BT from the long-term (1950-2005) average annual BT were calculated for each site in which at least 9 months of data were available. Monthly average bottom water temperatures (with stan- dard error of the mean) were calculated for each of 12 months for all sites. Monthly BT estimates from 1970 to 1983 (Delaware) and 1984 (Chesapeake Light,) use a single published monthly average (Table 2) while monthly BT estimates from NOAA buoys (Table 2) are averages calculated from hourly readings with n values >400 per month. Estuarine Habitats: Water temperature data col- lected at Public Landing, Maryland, in Chincoteague Bay from McGary and Sieling (1953) and Sieling (1957) were used to calculate average monthly water tempera- tures (with standard error of the mean) during the period 1951-1956. Modern (2001-2004) water temperature data from Chincoteague Bay stations south of Robins Marsh (XBM8149) (Figure 10, B2) and near the MD-VA border (XBM1301) (Figure 10, B3) were obtained from Page 152 THE NAUTILUS, Vol. 121, No. 3 ? (LI U c f- C? _>> * 12 O E 12 ? A. Delaware - Feb1961 -Dec1970 ? # - ? ? " ? - S ?m 4 - 9 ? 3 ~ 0 - e 9 e e 9 ? ? n = 75 102 138 170 148 183 162 164 119 127 78 101 - 1 1 1 1 1 1 1 1 1 1 1 1 1 10 11 12 '_ B. Chesapeake Jan 1958-Deci 971 ai en 5 ft s M e n = 202 9 0 d ? 9 188 174 180 169 186 145 197 184 125 159 e 173 T ~r 2 3 4 5 ?I 6 Month T T" 8 ~r 9 10 "T" 11 12 Figure 13. A\'erage monthly difference between sea surface temperature (SST) and bottom water temperature (BT) from lightship data (Table 2) for Delaware (A) and Chesapeake (B) lightships. Error bars indicate standard error of the mean. N \'alues (number of daily SST-BT pairs used to estimate the SST-BT differences) at each site are presented abo\'e the X axis in each panel. the Maryland Department of Natural Resources Eyes on the Bay web site (http://mddnr.chesapeakebay.net/ bay_cond/). Water depth at all (historical and modern) of these Chincoteague Bay sites is 3 m or less. Selling (1954) reported less than a degree Celsius variation be- tween surface and bottom water temperatures and the data presented in McGary and Seiling (1953) and Seiling (1957) from sites throughout the estuary confirm the well-mixed nature of these non-channel habitats. The Virginia Institute of Marine Science (VIMS) at Gloucester Point, Virginia (Figure 10, B4) maintained a water temperature monitoring station from 1986 through September 18, 2003, (arrival of Hurricane Isabel) that recorded bottom water temperatures (?C) at a depth of 2-3 m. The VIMS MoUuscan Ecology program has main- tained an environmental monitoring station located within 200 m of the original VIMS station since January 2005 that records bottom water temperatures (?C) at depths of 2 m. Hourly water temperature averages were obtained from the VIMS data archive (1986 to Sep 2003) and the VIMS MoUuscan Ecology program en\dronmen- tal data archive (2005) and used to calculate monthly residuals from the 1986-2005 mean and average monthly bottom water temperatures (with standard error of the RESULTS Morphology: The southern oyster drill specimens col- lected from the James River in February 2005 ranged in size from 66.4 to 75.6 mm shell length with an average shell length of 69.0 ? standard error of the mean 2.21 mm. Southern oyster drills collected in April 2006 from Back River had shell lengths ranging from 73 to 89 mm with an average shell length of 83.3 ? 1.41 mm. Size frequency distributions for both collections (Figure 11) indicate that all specimens were adults (sexually mature; Burkenroad, I93I; Butler, 1985) and represent the up- per end of the size distribution typically found in Loui- siana habitats (Brown and Richardson, 1987; Brown et al., 2004). These large individuals have probably reached a size refuge from most local predators including blue crabs (Callinectes sapidus, see the work of Turra et al., 2005, using C. danae preying on S. haemastoma) and are certainly capable of eating oysters >50 mm shell height (Garton, 1986; Brown and Richardson, 1987). J. M. Harding and M. G. Harasewych, 2007 Page 153 6 4 2 ^?^ ? 0 m ? c -2 ? i_ ? -4 !)= X} -6 <^ y^ w w >> 6 s: c 4 o F / ? ai 0 0) ^ -2 -4 = A. Delaware - r Jan 1984- Dec 2005 ~ ? ? ^ ^ e e ~ " r 0 ? 0 0 : j- 0 - :n= 10261 10050 12342 11677 12880 12493 13543 14049 12911 13168 11876 11666 ~ 1 1 1 1 1 1 1 1 1 1 1 i -6 - B. Chesapeake Jan 1985-Dec 2005 m 0 0 0 e 0 m 0 0 0 n=10317 9982 11059 10995 11258 12175 12422 11965 11671 11434 12023 11430 - T 2 T T 4 T 6 Month T 9 T 10 T 11 T 12 Figure 14. Average monthly difference between sea surface temperature (SST) and air temperature (AT) from NOAA buoys (Table 2) for Delaware (A) and Chesapeake (B) buoys. Error bars indicate standard error of the mean. N values (number of daily SST-AT pairs used to estimate the SST-AT differences) at each site are presented above the X axis in each panel. DNA Bar-coding: Comparisons of a 591 base-pair portion of the mitochondrial gene for cytochrome c oxi- dase I from the samples listed in Table 1 revealed that specimens of Stramonita collected within Chesapeake Bay were genetically very similar or identical to speci- mens collected from Fort Pierce Inlet, on the southeast- ern coast of Florida, but differed substantially from Azorean samples and even more so from specimens from the northwestern coast of Florida (Table 3). Maximum parsimony analyses of the 144 phylogenetically informa- tive sites using the exhaustive search algorithm yielded four equally parsimonious trees (length = 176; ci = 0.955; ri = 0.960). Figure 12 illustrates strict consensus tree of these four trees, including results of bootstrap and jack- knife analyses. Water Temperature Data: COASTAL HABITATS: Aver- age annual bottom water temperatures in the period 1950-2005 for the two stations along the US Atlantic coast followed a latitudinal trend and were lower (9.81?C, SEM = 0.15, 600 months of data) at Delaware than at Chesapeake (12.30?C, SEM = 0.21, 596 months of data). The differences between SST and BT recorded by the lightships (approximately 1956-71) show the sea- sonal development of the thermocline at Delaware and Chesapeake beginning in April and persisting until Oc- tober with the most pronounced differences between surface and bottom water temperatures occurring in July and August (Figure 13) when these stations experience surface temperatures that are at least 7? C higher than bottom temperatures. Air temperatures recorded by the Delaware and Chesapeake NOAA buoys (Table 2) are 1-2? warmer than SST from April through July (Fig- ure 14). Examination of annual average residual bottom water temperatures from the average long-term (1950-2005) bottom water temperatures at coastal stations (Figure 15) shows that both sites experienced above average wa- ter temperatures during the late 1950s. Multiple con- secutive years between 1970-1980 and, again, in the pe- riod 1995-2005. Estimated bottom water temperatures for 2002 were among the highest obsen ed during the period 1950-2002 for Delaware and Chesapeake Bays. Delaware and Chesapeake, both north of Cape Hatteras, experience water temperatures of 10?C or less eight and ?ve months out of the year, respectively (Table 4A). Page 154 THE NAUTILUS, Vol. 121, No. 3 ? 0) Q. E -I?f E o a o ?? 0) O) m ] 0) in o o ^ o m os E p ?o en o:: A. Delaware mmkovjv? coco coco cor^r^h~r^r^oDCDCDCDCDcncncn ocnooo o I o. E m I o .o (U Q) 5 I ib' o o ?o 'i? 0? B. Chesapeake 2 _ '1 !.. -1 : -2 . OCM-* COODOCM-4-COODO CM-^COOOOtN-*COOO OM mioio toiocococococor-- r^h-r~-r~-oooooooooo (?> -* CO 00 o CM ?^ c? ?? c? o ?3 CD O? Oi O? o (M (M Year Figure 15. Annual average residual bottom temperature (BT) from the long term (1950-2005) average bottom temperatures for Delaware (A) and Chesapeake (B). Long term a\'erage data for each site are presented in Table 4. The error bars represent the standard error of the mean in degrees Celsius. J. M. Harding and M. G. Harasewych, 2007 Page 155 Table 4. Summary of average monthly bottom water temperatures for coastal stations (A, 1950-2005) and estuarine stations (B) shown in Figure 10 and discussed in text. Standard error of the mean for each value is given in parentheses. A. Month Delaware Chesapeake Jan 6.99 (0.22) 6.96 (0.24) Feb 5.25 (0.20) 5.60 (0.23) Mar 5.58 (0.18) 6.12 (0.21) Apr 7.02 (0.15) 8.31 (0.20) May 8.88 (0.22) 11.02 (0.21) Jun 9.33 (0.18) 14.34 (0.21) Jul 9.45 (0.15) 14.93 (0.20) Aug 10.69 (0.20) 16.72 (0.25) Sep 14.62 (0.29) 20.27 (0.20) Oct 16.56 (0.20) 19.11 (0.21) Nov 13.57 (0.15) 11.02 (0.21) Dec 10.06 (0.18) 10.41 (0.23) Chincoteague Bay, Chincoteague Bay, Chincoteague Bay, York River, Public Landing, MD XBM8149 XBM1301 Gloucester Point, VA Month (1951-56) (2001-04) (2001-04) (1986-2005) Jan 4.14 (0.36) 5.3 5.7 5.32 (0.36) Feb 6.22 (0.33) 5.25 5.38 5.55 (0.47) Mar 9.76 (0.35) 9.65 9.9 8.46 (0.33) Apr 14.57 (0.43) 15.53 15.85 13.61 (0.30) May 20.1 (0.38) 18.65 18.87 18.86 (0.26) Jun 24.08 (0.38) 27.25 26.33 23.91 (0.26) Jul 27.77 (0.32) 26.1 25.85 26.96 (0.18) Aug 27.2 (0.26) 27.42 27.65 27.11 (0.18) Sep 23.6 (0.40) 22.95 22.93 23.99 (0.59) Oct 17.32 (0.50) 14.55 14.6 19.28 (0.33) Nov 10.72 (0.38) 15.1 15.68 13.56 (0.24) Dec 5.64 (0.39) 5.2 5.5 8.36 (0.54) ESTUARINE HABITATS: Annual average residual bottom water temperatures from the York River at Gloucester Point, Virginia were higher than the 1986-2005 annual average (16.23 ? 0.56?C) in 10 of the 18 years for which data are available (Figure 16) including 2005. The relatively shallow (3 m or less) estuarine habitats examined in Chincoteague and Chesapeake Bays expe- rience a seasonal average monthly water temperature cycle appropriate for their latitudes (Table 4B) with wa- ter temperatures less than 10?C obser\'ed December through March. The same pattern was obsen ed in Chin- coteague Bay during 1951-1956 (Table 4B). DISCUSSION The collections of live Stramonita from the James River (2005) and Back River (2006) mark the first modern record of this predatory gastropod from the Chesapeake Bay interior. The morphology of these specimens, par- ticularly their larger size, deeply channeled suture, and the presence of strong, rugose shoulder nodules in the Back River samples, is similar to that of some populations from the northern Gulf of Mexico [particularly the form named Stramonita haemastoma haysae (Clench, 1927) (Figure 8)]. This, in turn, has raised questions about whether these animals were introduced into Chesapeake Bay, and the possible source of such introductions. How- ever, the results of the "DNA bar-coding" study clearly indicate that the Chesapeake Stramonita haemastoma floridana are genetically very similar to, and in one case indistinguishable from, a population of Stramonita hae- mastoma floridana from southeastern Florida. There seems little doubt that the source of the Chesapeake Stramonita haemastoma floridana populations lies along the eastern seaboard of the United States. Less clear is whether these populations were introduced into Chesa- peake Bay with oysters, as had been suggested by Sieling (1960) for the Chincoteague Stramonita, or if their pres- ence is due to a northward expansion of the range of Stramonita haemastoma floridana due to warmer ocean temperatures. The "bar-coding" data also indicate that the east-coast Stramonita are well differentiated from, yet more similar to Stramonita from the Azores than to specimens from the northern Gulf of Mexico (Figure 12). Pro\'isional taxonomic consequences of this study are to recognize the Azorean populations as Stramonita haemastoma hae- mastoma, to retain the usage of Stramonita haemastoma Page 156 THE NAUTILUS, Vol. 121, No. 3 ? ? 4-1 0) Q. E E ? o O) 2 I to l?i o o (N CO on *^- ? P CO York River, Gloucester Point, VA 2 1 1 1 -1 1 -2 1 10 10 S tD 00 o CM ID m CD fO 3 to 00 o ?M CD (D h~ r- ?if CD 00 o CM r^ r- r- 00 00 o>a>o>o> o)o>o)o>o)o>o)o>o)o>o)o>a)o> Year Figure 16. Annual average residual bottom temperature (BT) from the long term (1986-2005) average bottom temperatures (16.23 ?C, standard error of the mean 0.56 ?C) recorded at Gloucester Point, Virginia. The error bars represent the standard error of the mean in degrees Celsius. floridana for the populations from the eastern United States, and to distinguish the populations from the north- ern Gulf of Mexico as Stramonita canaliculata, as advo- cated by Liu et al. (1991). Detailed analyses of this spe- cies complex over its entire geographic range are clearly required to better understand its biogeography and tax- onomy. Water temperature patterns in Chesapeake Bay habi- tats and along the coast of the lower Mid-Atlantic Bight since the late 1990s have been above average as were water temperature trends obser\'ed in the period 1955- 1957 when live Stramonita were collected on the Atlantic coast of Maryland and Virginia (Sieling, 1960). Although the lower thermal lethal limit for southern oyster drills is unknown, the fact that observed seasonal trends in bot- tom water temperatures in Chincoteague Bay during the period 1951-56 are within 1 to 2?C of bottom water temperatures observed in the lower York River from 1986-2005 suggests that Chesapeake Bay tributaries may have seasonal temperature regimes conducive to survival of this animal. Living southern oyster drills were col- lected from Chincoteague Bay by Prezant et al. (2002) during surveys between 1991 and 1996, some 40 years after the initial collection (Sieling, 1956). The very low population levels ("rare". Counts and Bashore, 1991) of southern oyster drills obsen'ed in Chincoteague Bay since 1988-89 have been attributed to a decline in local oyster resources (Counts and Bashore, 1991) rather than unfavorable water temperatures. The tributaries of the lower Chesapeake Bay have resident populations of bi- valves commonly eaten by Stramonita including mussels and oysters (Butler, 1985; Carton, 1986; Brown and Ri- chardson, 1987). Several of these tributaries are also sites of focused oyster restoration efforts that incorporate ad- dition of either spat on shell or broodstock oysters fur- ther expanding the potential prey field for southern oys- ter drills. If these collections represent the beginning of an in- vasion into Chesapeake Bay tributaries, the persistence o? Stramonita haemastonia floridana in Chesapeake Bay will be dictated by thermal tolerances as subjected to seasonal temperature cycles, while the geographic distri- bution will be set by salinity tolerances of adult and lar- vae. Adult Stramonita haemastoma may survive at salini- ties as low as 5-7 ppt (Gunter, 1979; Stickle, 1999). Stra- monita haemastoma egg capsules survive and release viable larvae at salinities down to at least 7.5 ppt and possibly as low as 3.5 ppt (Stickle, 1999). Veliger lanae may survive up to 5 days when exposed to 10 ppt (Wells, 1961) but Roller and Stickle (1989) reported very low survival at salinities less than 15 ppt. In June 1972, heavy rains from Tropical Storm Agnes in combination with summer temperatures killed essentially all of the native oyster drills [Urosalpinx cin?rea (Say, 1822), Eupleura caudata (Say, 1822)] in the upper reaches of Chesapeake Bay tributaries and reset the distributional range of the J. M. Harding and M. G. Harasewych, 2007 Page 157 native drills to the high salinity, lower reaches of the Chesapeake Bay where natural oyster populations cur- rently persist in only limited regions and numbers. Re- establishment of these native gastropod species to their former habitats is occurring slowly over decadal time scales and is limited by the lack of planktonic larvae for both Urosalpinx cin?rea and Eupleura caudata. Reinva- sion of their historic habitats by the native drills is con- founded by the fact that the historically widespread dis- tribution of oyster reef habitat has been drastically re- duced in areal coverage since the early 1960s by a combination of diseases and en\ironmental degradation. Competition for the niche vacated by the native drills during Tropical Storm Agnes already includes one large non-native gastropod, the veined rapa whelk (Rapana venosa) (Harding and Mann, 1999; 2005), which has planktonic veliger lar\ ae like Stramonita haemastoma and is equally long-lived. Regardless of how these Stra- monita haemastoma got to these Chesapeake tributaries, the presence of adult individuals in these tributaries adds yet another competitor for this vacant niche as well as additional pr?dation pressure on local oyster resources. Successful invasion of a habitat requires a breeding population and the presence of all life history stages in the new habitat (Williamson, 1996). Southern oyster drills live from 5-20 yr in Florida (Butler, 1985) with generation times on the order of 12 months (Burken- road, 1931; Butler, 1954). The small number of speci- mens collected to date combined with the absence of these animals in annual fishery independent surveys of oyster reefs in the James River conducted by Harding and colleagues at VIMS and the Virginia Marine Re- sources Commission since the early 1990s may be an indicator that the observed southern oyster drills speci- mens represent small, isolated introductions that have not yet established local populations. ACKNOWLEDGMENTS Special thanks are extended to all local watermen who have donated Stramonita haemastoma and Rapana venosa to the VIMS research programs. Karen Caposella, Christina Conrath, Meredith Fagan, Adriana Picariello, and Matt Robinson assisted with local whelk collections by VIMS. Dr. Gregory Herbert, University of South Florida, kindly provided specimens of Stramonita from St. Andrew's Bay, Florida. Azorean specimens were col- lected during a workshop in Vila Franca do Campo, Sao Miguel, Azores, hosted by The University of the Azores. The Workshop was a joint organization of Sociedade Afonso Chaves and the Department of Biology of the University of the Azores. Support from FLAD (Portu- guese-American Foundation for Development) is grate- fully acknowledged. Specimens from the Indian River Inlet were collected during a workshop supported by the Smithsonian Marine Station at Fort Pierce. 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