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SMITHSONIAN CONTRIBUTIONS TO ZOOLOGY • NUMBER 588

Anchialine Ostracoda (Halocyprididae) from San Salvador, Bahamas

Louis S. Kornicker
and Douglas J. Barr

SMITHSONIAN INSTITUTION PRESS

Washington, D.C.

1997

ABSTRACT

Kornicker, Louis S., and Douglas J. Barr. Anchialine Ostracoda (Halocyprididae) from San Salvador, Bahamas. Smithsonian Contributions to Zoology, number 588, 20 pages, 11 figures, 3 tables, 1997.—Spelaeoecia barri Kornicker, new species, an anchialine halocyprid Ostracoda (Halocyprididae: Deeveyinae), is described and illustrated from collections made at Dixon Hill Lighthouse Cave, San Salvador, Bahamas. Descriptions of adults and four juvenile stages are included and a discussion of ontogeny is given. A key to species of Spelaeoecia also is presented.

OFFICIAL PUBLICATION DATE is handstamped in a limited number of initial copies and is recorded in the Institution's annual report, Annals of the Smithsonian Institution. SERIES COVER DESIGN: The coral Montastrea cavernosa (Linnaeus).

Library of Congress Cataloging-in-Publication Data

Kornicker, Louis S., 1919-

Anchialine Ostracoda (Halocyprididae) from San Salvador, Bahamas/Louis S. Kornicker and Douglas J. Barr.

p. cm.—(Smithsonian contributions to zoology ; no. 588)

Includes bibliographical references (p. 20).

1. Ostracoda—Bahamas—San Salvador Island—Classification.

I.—Barr, Douglas J. II. Title. III. Series.

QL1.S54 no. 588 [QL444.08] 595.3′3—dc21 96-49163

The paper used in this publication meets the minimum requirements of the American National Standard for Permanence of Paper for Printed Library Materials Z39.48—1984.

Contents

Introduction

Description of Habitat

Other Biota in Cave

Methods

Disposition of Specimens

Abbreviations

Acknowledgments

Order HALOCYPRIDA Dana, 1853

Suborder HALOCYPRIDINA Dana, 1853

Superfamily HALOCYPRIDOIDEA Dana, 1853

Family HALOCYPRIDIDAE Dana, 1853

Subfamily DEEVEYINAE Kornicker and Iliffe, 1985

Spelaeoecia Angel and Iliffe, 1987

Key to the Species of Spelaeoecia (adults)

Spelaeoecia barri Kornicker, new species

Literature Cited

Requirements for Smithsonian Series Publication

Anchialine Ostracoda (Halocyprididae) from San Salvador, Bahamas

Louis S. Kornicker and Douglas J. Barr

____________

Louis S. Kornicker, Division of Crustacea, Department of Invertebrate Zoology, National Museum of Natural History, Smithsonian Institution, Washington, D.C. 20560. Douglas J. Barr, Biology Department, Schumucker Science Center, West Chester University, West Chester, Pennsylvania 19383.

Review Chairman: Austin B. Williams, National Marine Fisheries Service Systematics Laboratory, National Museum of Natural History, Smithsonian Institution.

Reviewers: Martin V. Angel, Institute of Oceanographic Sciences, Deacon Laboratory, Wormley, Godalming, United Kingdom; Dan L. Danielopol, Institut für Limnologie, Mondsee, Austria.

Introduction

DESCRIPTION OF HABITAT.—San Salvador Island (24°N, 74°25′W) is located on the eastern edge of the Bahama Platform, approximately 550 km east of Miami, Florida. Dixon Hill Lighthouse Cave is on the northeastern tip of the island, about 800 m inland, without an obvious connection to the sea. The cave has water-filled passages up to 2 m in depth, with a tidal fluctuation of up to 60 cm. The salinity remains steady at 35 ppt, although the warmer coastal water of San Salvador ranges from 37 to 39 ppt. The water temperature in the cave is approximately 25°C in the summer, slightly cooler than the 30°C of the surrounding coastal water. Dissolved oxygen tests indicate a fairly stable 8–10 ppm year-round.

The water-filled passages of Lighthouse Cave contain a rich organic flocculent layer, up to one foot in depth, that blankets the cave floor. It is composed primarily of bat guano, but it also includes allocthonous material carried in by the tide or washed down by rainwater through a collapsed modified solution pit that enters the cave's large central room.

Observations of the junior author suggest that the new species of ostracode described herein, Spelaeoecia barri, dwells in the flocculent layer. Individuals swam out of the layer and into the water above only when the floc was disturbed by the collector. They would spend only a brief time swimming, before darting back to the safety of the organic layer. A similar behavior has been noted previously in Lighthouse Cave for the isopod Bahalana geracei (Carpenter, 1981).

OTHER BIOTA IN CAVE.—Biological expeditions to Lighthouse Cave since the mid 1970s have resulted in the description of two new isopods, Bahalana geracei Carpenter, 1981, Neostenetroides stocki Carpenter and Magniez, 1982; three new sponges, Pellina pencilliformis, Prosuberites geracei, and Cinachyra subterranea Van Soest and Sass, 1981; and a new genus and species of a demersal copepod, Enantiosis cavernicola Barr, 1984. The pink cave shrimp Barbouria cubensis (von Martens, 1872) is common. The nonaquatic species include bats, land crabs (Gecarcinus sp.), cockroaches, and pseudo-scorpions.

METHODS.—With the exception of one juvenile collected in 1986 by Thomas M. Iliffe, specimens were collected by the junior author with baited traps between 28 to 31 January 1984. The traps were constructed using one-quart plastic bottles with the bottom cut off and replaced with a fine-meshed Nitex bolting cloth affixed with a rubber band to allow for better water flow. The cap of the bottle was replaced with a plastic funnel, six inches in diameter. The funnel was glued in place. The purpose of the funnel was to provide a wide opening to guide predators to the bait. The trap was then attached to a small rubber ball on the surface via a nylon line so it could be retrieved easily in the dark, murky water. The bait was pieces of the pink cave shrimp Barbouria cubensis, which could be easily captured in the cave. The traps were left in the cave for 24 hours before retrieval.

DISPOSITION OF SPECIMENS.—All specimens examined by the senior author have been deposited in the National Museum of Natural History, Smithsonian Institution, and have been assigned USNM catalog numbers.

ABBREVIATIONS.—In the figures, Arabic numerals indicate limbs 1–7, as well as individual joints of each limb (the location of the numeral indicating whether a limb or joint is indicated). Roman numerals I—III indicate the endites. Arrows indicate anterior.

The following abbreviations are used in the illustrations and legends.

am

central adductor muscle attachments

ant

antenna

Bo

Bellonci organ

bas

basale

co

copulatory organ

cx

coxale

e

valve edge

end

endopodite

epip

epipodite

esop

esophagus

ex

exopodite

fu

furca

gen

genitalia

gl

gland

im

inner margin of infold

iv

inside view of valve

lft

left valve

ll

lower lip

Iv

lateral view

mnd

mandible

mv

medial view

nabs

not all bristles shown

ov

outside view of valve

oval

mandibular attachment to valve

precx

precoxale

prot

protopodite

rt, rv

right valve

ul

upper lip

up

unpaired bristle of furca

vv

ventral view

ACKNOWLEDGMENTS.—Collection of a specimen by Thomas M. Iliffe was supported by National Science Foundation Grants (BSR 8215672 and BSR 8417494) to Thomas M. Iliffe. We are indebted to the College Center of the Finger Lakes Bahamian Field Station and its staff on San Salvador Island for logistical support in the Bahamas to both Barr and Iliffe. We also thank Jack R. Schroeder, Jack Schroeder Associates, and Molly K. Ryan, Smithsonian Institution, for inking shell and appendage drawings; Elizabeth Harrison-Nelson, Smithsonian Institution, for general assistance; and Diane M. Tyler, Smithsonian Institution Press, for final editing and preparation of the manuscript for publication. The description of the new species is solely the work of the senior author, who named it for the junior author.

Order HALOCYPRIDA Dana, 1853

Suborder HALOCYPRIDINA Dana, 1853

COMPOSITION.—The suborder comprises the superfamilies Halocypridoidea Dana, 1853, and Thaumatocypridoidea Müller, 1906. Only the former is represented in the collections reported upon herein.

Superfamily HALOCYPRIDOIDEA Dana, 1853

COMPOSITION.—The superfamily includes the single family Halocyprididae Dana, 1853.

Family HALOCYPRIDIDAE Dana, 1853

COMPOSITION.—The family comprises five subfamilies of which only the Deeveyinae Kornicker and Iliffe. 1985, is represented in present collections.

Subfamily DEEVEYINAE Kornicker and Iliffe, 1985

COMPOSITION.—The subfamily comprises the genera Deeveya Kornicker and Iliffe, 1985, and Spelaeoecia Angel and Iliffe, 1987, of which only the latter is described herein. One specimen, collected by the junior author in a baited trap from the cave on 30–31 Jan 1984, was identified as Deeveya sp. by the senior author, but the specimen was lost subsequently. That specimen was collected with a specimen of S. barri, indicating that both species occur together.

Spelaeoecia Angel and Iliffe, 1987

Spelaeoecia Angel and Iliffe, 1987:545, figs. 2–6.

TYPE SPECIES.—Spelaeoecia bermudensis Angel and Iliffe, 1987:545.

COMPOSITION AND DISTRIBUTION.—The genus includes seven species from anchialine caves: Bermuda: S. bermudensis Angel and Iliffe, 1987. Bahamas: S. capax, S. sagax, S. styx Kornicker in Kornicker et al., 1990, and S. barri Kornicker, new species, herein. Jamaica: S. jamaicensis Kornicker and Iliffe, 1992. Cuba: S. cubensis Kornicker and Yager, 1996.

Key to the Species of Spelaeoecia

(adults)

Spelaeoecia barri Kornicker, new species

FIGURES 1–11

ETYMOLOGY.—Named in honor of Douglas J. Barr who collected most of the specimens upon which the species is based.

HOLOTYPE.—USNM 194399, undissected adult male in alcohol.

TYPE LOCALITY.—Dixon Hill Lighthouse Cave, San Salvador, Bahamas.

PARATYPES.—Baited trap 28–29 Jan 1984: Adult females: USNM 194323; USNM 194395H; USNM 194395J; USNM 194395L; USNM 194395N, 4 specimens; USNM 194395P, 3 specimens; USNM 194395Q, 3 specimens; USNM 194395R, 3 specimens; USNM 194395T, 2 specimens; USNM 194398. Adult males: USNM 194387; USNM 194396; USNM 194397; USNM 194395P, 2 specimens; USNM 194395Q; USNM 194395R, 2 specimens; USNM 194395T, 2 specimens; USNM 194401. A-1 females: USNM 194389; USNM 194390; USNM 194395C; USNM 194395F; USNM 194395G; USNM 194395M; USNM 194395Q. A-1 males: USNM 194390; USNM 194395E. A-2 females: USNM 194388; USNM 194395B; USNM 194395E. A-2 males: USNM 194395A; USNM 194395D. A-3 instars, sex unknown: USNM 194391; USNM 194392; USNM 194393; USNM 194394. A-4 instar, sex unknown: USNM 194400.

Baited trap 30–31 Jan 1984: A-1 male, USNM 194395K.

Plankton net, 13 Jul 1986: A-3 instar, sex unknown: USNM 194324.

DISTRIBUTION.—Dixon Hill Lighthouse Cave, San Salvador, Bahamas; collected in Jan 1984 and Jul 1986.

DESCRIPTION OF ADULT FEMALE (Figures 1–5).—Carapace elongate, dorsal margin straight, ventral margin broadly rounded; anterior incisur dorsal to midheight (Figure 1a–e). Anterior of valve viewed from inside with edge of valve indented at incisur (Figure 1f); anterior part of rostrum broadly overreaching edge of valve and with tapered tip (Figure 1f). In lateral view, posterodorsal corner of each valve broadly rounded and with considerably posterior projection; minute triangular process with small terminal indentation on dorsal edge of left valve (Figure 1e); and minute indentation posterior to glandular openings at corner of right valve (Figure 1g), with small bristle at anterior end of indentation (Figure 1d,g).

Ornamentation: Surface with abundant shallow pits (representative pits shown in Figure 1a–e), few scattered long bristles, and minute bristles along valve edge (Figure 1b,g).

Infold: Broad infold along free margin, broadest along anteroventral margin of valve (Figure 1a,f,g). List forming narrow bar posterior to posterior juncture, then extending ventrally as narrow ridge intersecting posterior edge of valve near midheight (Figure 1a,d,g); posterior infold of right valve with row of minute crenulations anterior to dorsal part of list (Figure 1d,g). Narrow anteroventral and ventral list present at midwidth of infold and terminating dorsal to midheight near midwidth of posterior infold.

Central Adductor Muscle Attachments (Figure 1h): 4 closely spaced ovoid attachments.

Glands: Posterodorsal corner of right valve with minute glandular openings adjacent (anterior) to minute indentation (Figure 1d,g); minute bristle at posterior end of glandular process of right valve (Figure 1d,g). Posterodorsal corner of left valve with minute triangular process (no internal glandular structures evident) (Figure 1e). Short ducts leading to small external pores visible along ventral and anteroventral edges of valve (Figure 1f); 1 or 2 similar ducts on posterodorsal infold (Figure 1g), but whether they exit on infold or on outer valve surface was not resolved.

Selvage: Indistinct broad striated lamellar prolongation along valve edge medial to inner surface of rostrum (Figure 1f).

Carapace Size (length, height in mm): USNM 194323, 1.32, 0.76. USNM 194395H, 1.32, 0.80. USNM 194395J, 1.27, 0.80. USNM 194395L, 1.31, 0.76. USNM 194395N, 4 specimens: 1.30, 0.77; 1.32, 0.75; 1.25, 0.72; 1.22, 0.71. USNM 194395P, 3 specimens: 1.25, 0.69; 1.23, 0.70; 1.28, 0.75. USNM 194395Q, 3 specimens: 1.26, 0.76; 1.24, 0.72; 1.29, 0.75. USNM 194395R, 3 specimens: 1.29, 0.75; 1.30, 0.76; 1.24, 0.76. USNM 194395T, 2 specimens: 1.22, 0.69; 1.27, 0.77. USNM 194398, left valve (posterior of right valve missing), 1.24, 0.75.

First Antenna (Figure 2a,b): With 8 joints, but suture separating joints 3 and 4 indistinct away from ventral and dorsal margins. 1st joint with minute distal lateral and medial spines; terminal ventral lobe present but not well developed. 2nd joint with distal medial spinules and somewhat translucent spinous dorsal bristle. 3rd joint shorter than 2nd joint, bare. 4th joint about one-half length of 3rd joint, with terminal dorsal bristle. 5th joint with long ventral filament with minute, widely separated marginal spines. 6th joint bare. 7th joint with short spinous ringed a-bristle and with ventral medial b-bristle about length of stouter ventral c-bristle; both b- and c-bristles filament-like with minute, widely separated marginal spines and with terminal papillae. 8th joint small with 4 bristles: dorsal medial d-bristle filament-like, about twice length of a-bristle; long lateral e-bristle longer and stouter than d-bristle, with indistinct proximal rings and minute, widely separated marginal spines (tip missing from both limbs of USNM 194323); f-bristle medial to e- and g-bristles, shorter than e-bristle, either not oriented ventrally or only slightly ventrally, and filament-like with terminal papilla; lateral g-bristle filament-like, longer than f-bristle, with widely separated marginal spines and with terminal papilla.

FIGURE 1.—Spelaoecia barri Kornicker, new species, paratype, USNM 194323, adult female: a, complete specimen from right side showing representative pits, length 1.32 mm; b, posteroventral corner right valve, ov; c, anterior right valve, ov; d, posterodorsal corner of both valves from right side; e, posterodorsal corner left valve, ov; f, anterior right valve, iv; g, inside view of posterior of valves (not completely flattened); h, central adductor muscle attachments, left valve.

FIGURE 2.—Spelaeoecia barri Kornicker, new species, paratype, USNM 194323, adult female: a, right 1st antenna, lv; b, part of anterior of body from left side, lv; c, part right 2nd antenna, lv; d, endopodite left 2nd antenna, mv; e, endopodite left 2nd antenna, lv; f, tip exopodite right 2nd antenna, mv; g, coxale endite left mandible, lv; h, coxale endite right mandible, mv; i, part left mandible (nabs), mv; j, part right mandible, mv; k, part left mandible, mv; l, part left mandible, lv.

Second Antenna: Protopodite bare (Figure 2b,c). Endopodite 3-jointed but with 2nd and 3rd joints fused (Figure 2d,e): 1st joint with long slender spinous a- and b-bristles; 2nd joint with 3 bristles: minute lateral e-bristle near base of f-bristle (not shown in Figure 2d), and 2 long terminal filament-like f- and g-bristles; 3rd joint with equilength filament-like bare h-, i-, and j-bristles each with terminal papillae, and minute medial bristle at base of j-bristle (not shown in Figure 2d,e). Exopodite with 9 joints (joints 7–9 shown in Figure 2f): 1st joint divided into long proximal and short distal parts, with long terminal bristle with natatory hairs, and with ventral spines on left limb of USNM 194323 but not on right limb; bristles of joints 2–7 with natatory hairs; bristle of 2nd joint with ventral spines on left limb of USNM 194323 but not on right limb; bristle of 8th joint with natatory hairs and long distal dorsal spines; 9th joint with 4 bristles (1 minute dorsal bare, 1 short and 1 medium with dorsal spines but no hairs, 1 long ventral with natatory hairs and distal dorsal spines); all long bristle with 1–3 long proximal segments followed by closely spaced rings. (Exopodial joints 8 and 9 missing on left limb of USNM 194323, apparently lost while specimen was alive because wound seems sclerotized.)

Mandible: Coxale endite with proximal and distal sets of teeth separated by gap (Figure 2g,h); proximal set comprising 4 broad cusps plus small posterior triangular tooth; surface between cusps and just proximal to cusps with slender spines; 1 minute indistinct spinous bristle on corner just anterior to anterior cusp and another posterior to posterior cusp; 2 pectinate bristles adjacent to triangular tooth; distal set of teeth comprising 2 flat teeth, each with 7 or 8 cusps; 1 stout curved spinous process and 1 adjacent small spinous bristle proximal to flat teeth (Figure 2g). Basale (Figure 2i–l) with distal edge having 5 triangular cusps with minute proximal marginal teeth and 1 bare posterior triangular cusp set back from edge (Figure 2k); lateral surface near distal edge with sharp triangular tooth near midwidth (dashed in Figure 2k); lateral surface distal to midlength with 2 short and 4 longer bristles (none entwined); anterior margin with short proximal bristle (with base on medial side) and long bristle distal to midlength; posterior margin hirsute with 2 short distal ringed bristles (proximal with pointed tip, distal tubular); proximal medial surface with transparent plumose bristle on hirsute protuberance; 2 transparent plumose bristles on or close to dorsal margin (Figure 2i,l; only 1 shown in Figure 2k); distal medial surface with rows of hairs; lateral surface near insertion of endopodite with long bare bristle (Figure 2i,k,l). Endopodite (Figure 2l) with 1st joint having 3 distal bristles (1 long dorsal, 1 short ventral, 1 long medial near midwidth); 2nd joint widening distally, with medial hairs, 3 terminal dorsal bristles (1 long stout unringed spinous claw-like, 2 short ringed (1 lateral, 1 medial)), and 1 long terminal ventral bristle; 3rd joint with 7 bristles (2 long stout claw-like spinous bristles, 3 short spinous ringed bristles forming medial row near terminal edge, 1 short lateral bristle on ventral corner, and 1 longer ringed bristles on terminal lateral edge slightly inward from ventral margin); anterior and posterior margins and medial surface of joint hirsute.

Maxilla (Figure 3a–d): Endite I with 2 proximal and 11 terminal bristles (5 tubular) (Figure 3a); endite II with 2 proximal and 10 terminal bristles (5 tubular) (Figure 3a); endite III with 1 proximal and 6 terminal bristles (2 tubular) (Figure 3a). Coxale with stout plumose dorsal bristle (Figure 3c,d; bristle missing in Figure 3b). Basale partly fused to coxale with 1 short pointed bristle (missing on right limb of USNM 194323, Figure 3b) and 1 tubular ventral bristle (not shown in Figure 3c). 1st endopodial joint with 10–12 bristles (Figure 3b–d); 2nd joint with anterior hairs, 2 stout pectinate claw-like bristles, and 5 slender ringed bristles (Figure 3b–d).

Fifth Limb (Figure 4a,b): Epipodite with plumose bristles forming 3 groups (ventral group with 5 bristles, middle group with 6 bristles, dorsal group with 5 bristles (4 long, 1 short dorsal) (Figure 4b). Protopodite without glandular process and with 2 ventral endites (Figure 4a; not shown in Figure 4b): endite I with medial hairs and 4 bristles (2 ventral with long spines, 1 ventral tubular, and 1 proximal medial with short spines); endite II with 3 bristles (1 long lateral with long spines, and 2 shorter tubular bare and closer to ventral margin). Basale with medial hairs, 1 long lateral anterior bristle with long spines, 1 ventral endite with 1 short proximal medial bristle (with short spines) and 5 bristles closer to ventral margin (1 long and 1 short unringed, claw-like, and pectinate along posterior margins, and 3 tubular either bare or with short spines) (Figure 4a; not shown in Figure 4b). Endopodite with 1 short proximal medial bristle with short spines, and 10 additional bristles (1 short tooth-like medial subventral, 1 long bare (pointed) lateral subventral, 1 short lateral tubular proximal, 2 tubular ventral bare, 2 claw-like unringed ventral with posterior teeth, 2 anterior long with long spines, 1 long ringed proximal lateral with long spines) (Figure 4a; not shown in Figure 4b). Exopodite (Figure 4a,b): 1st joint: dorsal margin with 1 long bare terminal bristle and 1 plumose subterminal bristle; ventral margin divided into 2 parts by depression in ventral margin near midlength; proximal part with 3 bare ventral bristles, 1 long plumose lateral bristle near midwidth, and 1 fairly long distal medial bristle near ventral margin; distal part with 3 bare ventral bristles and 2 distal plumose lateral bristles, 1 near dorsal margin. 2nd joint: dorsal margin with 1 distal bristle; ventral margin with 4 slender bristles near midlength. 3rd joint with 2 stout claw-like bristles (dorsal with oblique lines, both with minute ventral spines), 1 slender ringed ventral bristle, and 1 minute indistinct spine-like medial bristle.

FIGURE 3.—Spelaeoecia barri Kornicker, new species, paratype, USNM 194323, adult female: a, endites of right maxilla;b, right maxilla (nabs), mv; c, right maxilla (not under cover slip, nabs), vv; d, left maxilla (nabs), vv; e, left 7th limb and genitalia;f, left lamella of furca; g, apron of furca from right side.

FIGURE 4.—Spelaeoecia barri Kornicker, new species, paratype, USNM 194323, adult female: a, left 5th limb (under cover slip), mv; b. left 5th limb drawn attached to body and not under cover slip (nabs), lv;c, left 6th limb drawn attached to body and not under cover slip (nabs), lv; d, left 6th limb (under cover slip), mv.

Sixth Limb (Figure 4c,d): Epipodite with plumose bristles forming 3 groups (ventral and middle group each with 5 bristles, dorsal group with 7 bristles (6 long, 1 short)) (Figure 4c). Protopodite separated from basale by suture and divided by indistinct suture into 2 parts interpreted to be precoxale and coxale, both with long medial hairs: precoxale with 4 bristles with long spines; coxale with 4 bristles (2 with long spines, 1 with long hairs, and 1 with short spines) (Figure 4d; not all bristles shown in Figure 4c). Basale with 5 or 6 plumose bristles (4 or 5 near ventral margin, 1 distal lateral near midwidth or close to dorsal margin) (Figure 4d). Endopodite well developed with 5 long bristles (3 medial plumose (hairs not shown), 2 lateral bare). Exopodite 3-jointed: 1st joint with 3 bare ventral bristles (left limb of USNM 194323 with additional lateral distal bristle with long proximal and short distal spines (Figure 4d; not all bristles shown in Figure 4c)); 2nd joint with 2 or 3 bare distal bristles (1 dorsal, 1 medial on left limb of USNM 194323, 2 ventral on right limb); 3rd joint with 3 long terminal bristles (middle bristle claw-like with ventral teeth; dorsal bristle slender, somewhat claw-like, with oblique rings (rings not shown); ventral bristle slightly slenderer than dorsal bristle, ringed (rings not shown), bare) and 1 minute medial indistinct spine-like bristle (Figure 4d; not shown in Figure 4c).

Seventh Limb (Figure 3e): Elongate with 3 bare terminal bristles (1 longer than others).

Furca (Figure 3f): Each lamella with 8 claws with minute teeth along posterior edges (teeth not shown); no claws with transverse lines; minute indistinct triangular process with blunt tip (glandular?) between claws 1 and 2 but much closer to claw 2; minute triangular process follows last claw. Bifurcate well-developed unpaired bristle with minute marginal spines just posterior to furca (Figure 3f). Apron present anterior to furca (Figure 3g).

Bellonci Organ (Figure 2b): Branching in distal , branches slightly unequal in length, each with broadly rounded tip.

Lips: 2 small triangular processes (1 on each side) proximally on anterior margin (stippled in Figure 5a,b,e). Upper lip terminating posteriorly in spinous bar (bottom of Figure 5c). Row of 4 or 5 small round processes visible on each side of anterior near triangular process (Figure 5a,b). USNM 194373 with small area with minute spines on each side of anterior of lip and proximal to triangular processes but precise structure not resolved (Figure 5a,b). Lower lip a triangular process (with spines) on each side of mouth (Figure 5d,e).

Genitalia (Figure 3e): A small process with terminal spine present on left side of body near base of 7th limb.

Gut Content: Gut of USNM 194323 with fragments that may be remnants of crustaceans.

DESCRIPTION OF ADULT MALE (Figures 6–8).—Carapace similar in shape, ornamentation, infold, and glands to that of adult female (Figure 6a,h).

Central Adductor Muscle Attachments: Consisting of 4 ovoid attachments (Figure 6a–c,i). An oval, forming base of attachment of a ligament extending ventrally from dorsal tip of coxale of mandible, present anterior to muscle attachments (Figure 6b,c,i).

Carapace Size (length, height in mm): USNM 194387, 1.23, 0.69. USNM 194396, 1.22, 0.73. USNM 194397, 1.22, 0.72. USNM 194395P, 2 specimens: 1.23, 0.69; 1.22, 0.70. USNM 194395Q, 1.23, 0.70. USNM 194395R, 2 specimens: 1.19, 0.70; 1.22, 0.73. USNM 194395T, 2 specimens: 1.21, 0.72; 1.21, 0.68. USNM 194399, 1.24, 0.72. USNM 194401, 1.17, 0.69.

First Antenna (Figure 6k,l): Similar to that of adult female except suture between 3rd and 4th joints well developed.

Second Antenna: Protopodite bare. Endopodite 3-jointed (Figure 7a–d): 1st joint with a-bristle and longer b-bristle, both with indistinct spines; 2nd joint with short slender d- and e-bristles, long filament-like f- and g-bristles (with indistinct widely separated minute marginal spines (spines not shown) and with minute indistinct peg at base of f-bristle (detail in Figure 7b)); 3rd joint elongate, with filament like h-, i-, and j-bristles and long terminal slightly sclerotized process with bulbous tip with 2 minute terminal spines and lateral node; sclerotized process of right limb longer than that of left limb (Figures 7c,d, 8np). Exopodite: bristles of 1st and 2nd joints of both limbs with ventral spines and natatory hairs, otherwise exopodite similar to that of adult female.

Mandible: Coxale endite similar to that of adult female except distalmost of distal set of teeth of USNM 194357 with 6–9 cusps (Figure 7e,f). Basale (Figure 7g,h,i,j) and endopodite (Figure 7k,l) similar to those of adult female.

Maxilla (Figure 7m): Endite I similar to that of adult female; endite II with 2 proximal and 8 terminal bristles (2 tubular); endite III with 1 proximal and 5 terminal bristles (2 tubular). Coxale with spinous dorsal bristle. Basale with 2 ventral bristles (1 long tubular, 1 short). 1st endopodial joint with 10 or 11 bristles; 2nd endopodial joint similar to that of adult female.

Fifth Limb: Epipodite with 5 bristles in ventral group, 6 in middle group, and 5 (4 long, 1 short) in dorsal group (Figure 8a). Protopodite with short glandular process (Figure 8a-c). Endites, basale, endopodite, and exopodite, in general, similar to those of adult female, except minute medial spine-like bristle of 3rd exopodial joint not observed with certainty (Figure 8c,d).

Sixth Limb: Epipodite with 5 bristles in ventral group, 6 in middle group, and 6 (5 long, 1 short) in dorsal group (Figure 8f). Basale with 7 plumose bristles (6 near ventral margin and 1 lateral near midwidth or close to dorsal margin). Endopodite similar to that of adult female (Figure 8g). Exopodite (Figure 8g): 1 st joint with 3 ventral bristles on both limbs; 2nd joint with 3 bristles (1 dorsal, 2 ventral); 3rd joint similar to that of adult female except minute medial spine-like bristle absent.

FIGURE 5.—Spelaeoecia barri Kornicker, new species, paratype, USNM 194323, adult female: a, anterior of body from right side; b, anterior view of body (slightly oblique); c, posterior view of upper lip; d, lower lips, vv; e, lower lip area of body from left side.

FIGURE 6.—Spelaeoecia barri Kornicker, new species, paratype, USNM 194387, adult male: a, complete specimen from left side showing some internal structures (dashed), length 1.23 mm; b,c, details from a; d, posterior right valve, ov; e, anterior part of flattened valves, iv; f, rostrum and incisur right valve, iv; g,h, posterodorsal corners of right and left valves, respectively, iv; i, oval attachment of coxale endite ligament and left ends of central adductor muscles, drawn from left side of animal removed from carapace; j, right 1 st antenna, lv; k, distal left 1st antenna, lv; l, Bellonci organ from right side.

FIGURE 7.—Spelaeoecia barri Kornicker, new species, paratype, USNM 194387, adult male: a, endopodite right 2nd antenna, mv; b, distal endopodite left 2nd antenna, mv; c,d, sclerotized process of 3rd joint of right and left 2nd antennae, respectively, mv; e,f, coxale endites of left (lv) and right (mv) mandibles, respectively; g, part of left mandible, lv; h, detail from g; i, dorsal end of basale of right mandible, mv; j, anterior of body from left side showing both right and left sclerotized triangular processes (stippled) (not under cover slip); k, endopodite left mandible, lv; l, distal part endopodite right mandible, mv; m, maxilla; n, ventral part of anterior of body showing sclerotized triangular process (stippled).

FIGURE 8.—Spelaeoecia barri Kornicker, new species, paratype, USNM 194387, adult male: a, proximal part of right 5th limb (nabs), lv; b, part left 5th limb (epipodial bristles not shown), lv; c, right 5th limb (not under cover slip; nabs), lv; d, part right 5th limb, mv; e, left 7th limb, lv; f, epipodite right 6th limb, lv; g, right 6th limb (spines not shown on endopodial bristles; not all epipodial bristles shown), lv; h, right furcal lamella and unpaired bristle; i, posterior of body from right side; j, copulatory organ from left side; k, tip of anterior process in j from left side (under cover slip); l, tip of anterior process in j from right side. Paratype, adult male, USNM 194396: m, copulatory organ from left side; n, sclerotized processes of 3rd endopodial joints of 2nd antennae. Holotype, adult male, USNM 194399: o, copulatory organ from left side; p, sclerotized processes of 3rd endopodial joints of 2nd antennae. Paratype, adult male, USNM 194401: q, copulatory organ from left side.

Seventh Limb (Figure 8e), Furca (Figure 8h), Upper and Lower Lips (Figure 7n): Similar to those of adult female.

Bellonci Organ (Figure 6l): Tips of branches more pointed than those of adult female.

Genitalia (Figure 8i–m,o,q): Copulatory organ consisting of 2 parts: anterior part with sclerotized triangular tip and tubular process; posterior part with broad spinous tip. Copulatory organ located left of apron of furca and right of left 5th and 6th limbs.

Gut: With minute unidentified particles.

DESCRIPTION OF A–1 FEMALE (Instar VI?) (Figure 9a–d).—Carapace similar to that of adult female (Figure 9a,b).

Central Adductor Muscle Attachments (Figure 9a,d): Consisting of 3 or 4 oval attachments (2 or 3 large, 1 small). Oval mandibular attachment similar to that of adult female.

Carapace Size (length, height in mm): USNM 194389, 1.08 mm, 0.67 mm. USNM 194390, 1.05; 0.60. USNM 194395C, 1.02, 0.59. USNM 194395F, 1.03, 0.65. USNM 194395G, 1.07, 0.65. USNM 194395M, 1.08, 0.63. USNM 194395Q, 1.11, 0.63.

Furca (Figure 9c): Similar to that of adult female except with only 7 claws.

Remaining Appendages and Upper Lip: Not examined in detail but, in general, similar to those of adult female.

Genitalia: Absent.

DESCRIPTION OF A–1 MALE (Instar VI?) (Figure 9e–i).—Carapace similar to that of adults and A–1 female (Figure 9e).

Carapace Size (length, height in mm): USNM 194390, 1.05, 0.60. USNM 194395E, 1.08, 0.64. USNM 194395K, 1.07, 0.62.

First Antenna (Figure 9f, nabs): Similar to that of adult female.

Second Antenna: Protopodite bare (Figure 9f). Endopodite 3-jointed but with 2nd and 3rd joints fused (Figure 9g): 1st joint with spinous a- and b-bristles; 2nd joint with stout g-bristle with indistinct widely separated spines, f-bristle thinner and shorter than g-bristle, with indistinct widely separated marginal spines and with minute lateral peg at base of f-bristle; 3rd joint with h-, i-, and j-bristles shorter than f-bristle, and with 2 minute bristles on small medial process at base of j-bristle. Exopodite 9-jointed: 1st joint divided into long proximal and short distal parts, with long terminal bristle with ventral spines; long bristle of 2nd joint with ventral spines and few distal dorsal natatory hairs; bristles of joints 3–8 with natatory hairs, no spines; 9th joint with 4 bristles (1 minute bare, 2 short with dorsal spines, 1 long with natatory hairs).

Mandible: Similar to that of adult female.

Maxilla, Fifth and Sixth Limbs: Not examined in detail but, in general, similar to those of adult female.

Seventh Limb: Similar to that of adult female.

Furca (Figure 9h,i) and Bellonci Organ (Figure 9f): Similar to those of A–1 female.

Genitalia (Figure 9h,i): Consisting of 2 lobes: anterior lobe broad with rounded tip (may have 3 subterminal bristles, but not clearly resolved (could be wrinkles) and not shown); posterior lobe narrow with rounded tip bearing 3 small spines. Both lobes on left side of apron of furca.

DESCRIPTION OF A–2 FEMALE (Instar V?) (Figure 9j–m).—Carapace similar to that of adult female (Figure 9j–l).

Central Adductor Muscle Attachments: Consisting of about 6 small oval attachments.

Carapace Size (length, height in mm): USNM 194388, 0.86 mm, 0.51 mm. USNM 194395B, 0.89, 0.51. USNM 194395E, 0.85, 0.55.

Furca (Figure 9m): Similar to that of adult female except with only 6 claws on each lamella.

Remaining Appendages and Lips: Not examined in detail but, in general, similar to those of adult female. 1 st endopodial joint of 2nd antenna with 2 dorsal bristles.

Genitalia: Absent.

DESCRIPTION OF A–2 MALE (Instar V?) (Figure 9n–p).—Carapace similar to that of adult female (Figure 9n).

Central Adductor Muscle Attachments (Figure 9n, o): Consisting of 5 or 6 small oval attachments; mandibular oval present anterior to central adductor muscle attachments (Figure 9o).

Carapace Size (length, height in mm): USNM 194395A, 0.84, 0.49. USNM 194395D, 0.88, 0.52.

Furca: Similar to that of A–2 female.

Remaining Appendages and Lips: Not examined in detail but, in general, similar to those of A–2 female.

Genitalia (Figure 9p): Consisting of 2 adjacent elongate lobes on left side of body: anterior lobe bare (not observed on USNM 194395A); posterior lobe with 2 small spine-like bristles.

DESCRIPTION OF A–3 INSTAR (Instar IV?) (sex unknown) (Figure 10a–l).—Carapace similar in shape, ornamentation, and selvage to those of adults (Figure 10a,b,d,e,h,i,k).

Central Adductor Muscle Attachments (Figure 10a,c,l): Comprising 7 or 8 small ovoid attachments. Large translucent mandibular oval present anteroventral to adductor attachments.

FIGURE 9 (opposite page).—Spelaeoecia barri Kornicker, new species, paratype, USNM 194389, A–1 female: a, complete specimen from left side, length 1.08 mm; b, detail from a; c, left lamella of furca. Paratype, USNM 194395F, A–1 female: d, central adductor muscle attachments of left valve (magnification 4 times that of a), ov. Paratype, USNM 194390, A–1 male: e, complete specimen from left side, length 1.05 mm; f, dorsal part of anterior of body from left side (nabs); g, endopodite of right 2nd antenna (only proximal parts shown of long bristles of 2nd and 3rd joints), mv; h, ventral part of posterior of body from left side; i, copulatory organ and left lamella of furca (not all claws shown) from right side. Paratype, USNM 194388, A–2 female: j, complete specimen from left side showing outline of area containing adductor muscle attachments; k, dorsal view of j with valves partly open; l, detail from j showing representative shallow surface pits; m, left lamella of furca. Paratype, USNM 194395D, A–2 male: n, complete specimen from left side, length 0.88 mm: o, central adductor muscle scars and mandibular oval of left valve drawn at twice magnification of n, ov; p, tip of copulatory organ from left side drawn at 4 times magnification of n.

FIGURE 10 (opposite page).—Spelaeoecia barri Kornicker, new species, paratype, USNM 194324, A–3 instar: a, complete specimen from right side, length 0.69 mm; b, posterior of complete specimen from right side showing representative pits; c, detail from a of central adductor muscle attachments and mandibular oval; d, anterior left valve, iv; e, inside view of posterior ends of flattened valves; f right lamella of furca; g, 5th and 6th limbs shown attached to right side of body (nabs). Paratype, USNM 194391, A–3 instar (sex unknown): h, complete specimen from right side, length 0.68 mm; i, ventral view of h showing central adductor muscle (valves partly open); j 5th, 6th, and 7th limbs shown attached to right side of body as seen through right valve. Paratype, USNM 194392, A–3 instar (sex unknown): k, complete specimen from left side, length 0.71 m. Paratype, USNM 194393, A–3 instar (sex unknown): l, central adductor muscle attachments and mandibular oval of right valve, ov. Paratype, USNM 194400, A–4 instar (sex unknown): m, complete specimen from left side, length 0.55 mm; n, 5th and 6th limbs drawn attached to left side of body (nabs); o, left furcal lamella.

Glands (Figure 10b,e): Similar to those of adult female.

Carapace Size (length, height in mm): USNM 194324, 0.69, 0.41. USNM 194391, 0.68, 0.41. USNM 194392, 0.71, 0.44. USNM 194393, 0.67, 0.42. USNM 194394, 0.68, 0.41.

First Antenna, Mandible, Maxilla, and Bellonci Organ: Not examined in detail but all well developed. One branch of Bellonci organ slightly longer than the other as on adult female.

Second Antenna: 1st joint of endopodite with only 1 dorsal bristle; remaining part of endopodite not studied in detail but, in general, similar to that of adult. Protopodite and exopodite also not studied in detail but similar to those of adult (number of bristles on 9th joint of exopodite not determined).

Fifth and Sixth Limbs (Figure 10g,j): 6th limb extending well past 5th limb.

Seventh Limb: Absent on USNM 194324, 194393, and 194394. Well developed on USNM 194391 and 194392.

Furca (Figure 10f): Each lamella with 5 claws followed by small triangular tooth-like process. Minute triangular process between claws 1 and 2 but closer to claw 2. Divided unpaired bristle just posterior to furca (illustrated furca has aberrant single bristle). Apron present.

Genitalia: Absent.

Remarks: Five specimens in the collection are interpreted to be the A–3 instar based on their size (length 0.67–0.71 mm) and number of furcal claws (5). Two of the specimens have well-developed 7th limbs, whereas the limb is absent on 3 specimens. On all specimens the 6th limb extends posteriorly well past the 5th limb. The difference in development of the 7th limb on the specimens is interpreted to be the result of intraspecific variability.

DESCRIPTION OF A–4 INSTAR (Instar III?) (sex unknown) (Figure 10m–o).—Carapace similar to that of adult female (Figure 10m).

Carapace Size (length, height in mm): USNM 194400, 0.55, 0.34.

First and Second Antenna, Maxilla, Mandible, and Lips: Not examined in detail but, in general, similar to those of adult female. 1st endopodial joint of 2nd antenna with 1 dorsal bristle.

Fifth and Sixth Limbs (Figure 10n): Not examined in detail but 6th limb not extending past 5th limb.

Furca (Figure 10o): Similar to that of A–3 instar except with 4 furcal claws. Unpaired bristle divided.

Bellonci Organ: Similar to that of adult female.

Genitalia: Absent.

ONTOGENY.—The collection from San Salvador contains instars A–4 to the adult (Figure 11). If the species has six juvenile stages, the stages collected probably represent instars III, IV, V, VI, and adult. The 6th limb bears bristles on instar III, but it does not extend past the 5th limb. The 7th limb is absent on instar III and also on some specimens of instar IV. Each lamella of the furca of instar III bears four claws followed by small triangular process, and one claw is added in each subsequent instar, with the adult bearing eight claws (Table 1). The small triangular process follows the claws on the adult as well as on the juveniles. Each furcal lamella of unknown instars I and II probably bears 2 and 3 claws, respectively, as well as a triangular process.

The average growth factor for specimens in the collection is 1.22. The growth factor between instar VI and the adult is 1.14 for the male and 1.20 for the female, which results in adult males being smaller than adult females, but with a slight overlap in lengths (Table 2).

The collection contains specimens captured in a baited trap, and most likely it does not represent the population structure outside the trap (Table 3). The collection contained fewer males than females, but whether this is the result of sampling error or fewer males being present in the cave is unknown; however, the data may suggest that adult males are less numerous than adult females. Collections of another species of the genus, Spelaeoecia bermudensis Angel and Iliffe, 1987, also had fewer adult males than females (Angel and Iliffe, 1987; Kornicker, 1989:313). The small number of early instars in the trap suggests that they are less mobile than adults.

COMPARISONS.—The 1st antenna of S. barri differs from that of S. bermudensis in having no bristles on the 3rd joint and no ventral bristle on the 4th joint, and from that of S. jamaicensis in not having a ventral bristle on the 4th joint. The carapaces of previously described species of Spelaeoecia have lineations, reticules, or are without surface ornamentation, whereas S. barri has shallow surface pits (some of the pits are illustrated in Figure 1a). The furca of S. barri has eight claws on each lamella compared to five for S. cubensis and seven for S. bermudensis and S. styx. The posterior branch of the copulatory organ of S. sagax, S. styx, and S. bermudensis has a narrow tip compared to a broad tip on S. barri. Spelaeoecia capax, which is known from only the carapace, is much larger than S. barri (length 2.49 mm compared to 1.17–1.32 mm).

TABLE 1.—Order of appearance of appendages of Spelaeoecia barri, new species (+ = present, – = absent), and number of furcal claws. Unlisted appendages already are present on the A–4 instar.

FIGURE 11.—Carapace length-height distribution of growth stages of Spelaeoecia barri Kornicker, new species.

TABLE 2.—Average shell lengths and calculated growth factors for males and females of Spelaeoeca barri, new species (nd = no data).

TABLE 3.—Number of males and females of Spelaeoecia barri, new species, in the collection.

Literature Cited

Angel, M.V., and T.M. Iliffe

1987.   Spelaeocia bermudensis New Genus, New Species, a Halocyprid Ostracod from Marine Caves in Bermuda. Journal of Crustacean Biology, 7:541–553.

Barr, D.J.

1984.   Enantiosis cavernicola, a New Genus and Species of Demersal Copepod (Calanoida: Epacteriscidae) from San Salvador Island, Bahamas. Proceedings of the Biological Society of Washington, 97(1):160–166.

Carpenter, J.H.

1981.   Bahalana geracei n. gen., n. sp., a Troglobitic Marine Cirolanid Isopod from Lighthouse Cave, San Salvador Island, Bahamas. Bijdragen tot de Dierkunde, 51(2):259–267.

Carpenter, J.H., and G.J. Magniez

1982.   Deux Asellotes Stygobies des Indes Occidentales: Neostenetroides stocki, n. gen. n. sp., et Stenetrium sp. Bijdragen tot de Dierkunde, 52(2):200–206.

Dana, J.D.

1853.   Tribe III: Cyproidea = Ostracoda. In Crustacea of United States Exploring Expedition during the Years 1838, 1839, 1840, 1841, 1842, under the Command of Charles Wilkes, U.S.N., with Atlas of 96 plates, 14(2):1277–1304, plates 90, 91. Philadelphia: C. Sherman.

Kornicker, Louis S.

1989.   The Adult Male of the Troglobitic Ostracode Spelaeocia bermudensis Angel and Iliffe, 1987, from an Anchialine Cave in Bermuda (Crustacea: Ostracoda: Halocypridoidea). Proceedings of the Biological Society of Washington, 102(2):313–323, figures 1–5.

Kornicker, Louis S., and Thomas M. Iliffe

1985.   Deeveyinae, a New Subfamily of Ostracoda (Halocyprididae) from a Marine Cave on the Turks and Caicos Islands. Proceedings of the Biological Society of Washington, 98(2):476–493, figures 1–13.

1992.   Ostracoda (Halocypridina, Cladocopina) from Anchialine Caves in Jamaica, West Indies. Smithsonian Contributions to Zoology, 530, 22 pages, 11 figures, 1 table.

Kornicker, Louis S., and Jill Yager

1996.   The Troglobitic Halocyprid Ostracoda of Anchialine Caves in Cuba. Smithsonian Contributions to Zoology, 580, 16 pages, 9 figures, 1 table.

Kornicker, Louis S., Jill Yager, and Dennis Williams

1990.   Ostracoda (Halocyprididae) from Anchialine Caves in the Bahamas. Smithsonian Contributions to Zoology, 495, 51 pages, 30 figures, 4 tables.

Müller, G.W.

1906.   Ostracoda. In Wissenschaftliche Ergnebnisse der Deutsche Tiefsee-Expedition…1898–1899, 8(2): 154 pages, 31 plates.

Van Soest, R.W.M., and D.B. Sass

1981.   Marine Sponges from an Island Cave on San Salvador Island, Bahamas. Bijdragen tot de Dierkunde, 51(2):332–344.

Von Martens, E.

1872.   Ueber Cubanische Crustaceen nach den Sammlungen Dr. J. Gundlach's [sic]. Archiv für Naturgeschichte, 38(1):77–147, 257, 258, plates 4, 5.

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